Composition comprising elderberry extract as effective component for preventing, treating, or alleviating male climacteric syndrome
A technology of western elderberry and active ingredients, which is applied in the field of compositions for the prevention, treatment or improvement of male climacteric syndrome, and can solve problems such as endangering the quality of life and erectile dysfunction
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Embodiment 1
[0047] Embodiment 1: the preparation of western elderberry extract
[0048] Add 10 times the solvent (1L) to 100g of the fresh fruit of the purchased western elderberry, and use a heating mantle equipped with a reflux cooling device to extract twice for 2 hours, and then use a metal detector to use a 10-25μm filter Filtration was performed twice. Each extract was concentrated to 6.0-8.0 brix% by a vacuum concentrator, lyophilized into powder, and stored at -20°C for future use. In this experiment, the powdered extract was dissolved in distilled water (D.W) in different concentrations and used.
experiment example 1
[0053] Experimental example 1: Confirmation of the intracellular testosterone secretion ability of the western elderberry extract
[0054] 1) Culture of testicular cells (leydig cells)
[0055] As the testicular cell (leydig cell) TM3 cell, the cell sold by American Type Culture Collection (ATCC) was used. Dulbecco Modified Eagle medium (DMEM, high glucose) mixed 1:1 with Ham's F-12K (Kaighn's) medium, supplemented with 2.5% fetal bovine serum (FBS), 5% horse serum and 1% penicillin- streptomycin medium in CO 2 Testicular cells were cultured in an incubator (Formascientific, Inc.). Observe under a microscope to confirm that the cells are stabilized and then used in the experiment.
[0056] 2) Evaluation of testosterone secretion in leydig cells
[0057] In a 24-well culture plate at 1 × 10 5 After dispensing TM3 cells, the concentration / well was stable for 24 hr. Afterwards, the medium was replaced with a serum-free medium, the extract was diluted by concentration (μg / mL...
experiment example 2
[0060] Experimental example 2: Confirmation of the testosterone secretion ability of Western elderberry extract from an animal model of male climacteric syndrome
[0061] 1) Summary of animal models and experiments of male menopause
[0062] After purchasing and domesticating 23-week-old male SD mice (Samtaco, Korea), when the weight of each mouse was about 510 g (6 months old), 9 mice were used in each test group, and the mice were purchased 1 week before the end of the test. 11-week-old male SD mice (Samtaco, Korea) were introduced and domesticated, and a group of 9 mice was formed at 12-week-old.
[0063] Take 6-month-old old mice as a control group, and design the following experimental groups: For old mice, 300mg / kg Western Elderberry hot water extract was orally administered once a day, 7 times a week, for 12 weeks, to form The group used to confirm the improvement of andropause.
[0064] 2) Evaluation of serum testosterone secretion capacity
[0065] In order to anal...
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