Method for promoting nodulation and nitrogen fixation of root system of tumorous stem mustard by using azorhizobium caulinodans
A technology of nitrogen-fixing rhizobia and stem mustard, applied in horticultural methods, botany equipment and methods, applications, etc., to achieve great application value, increase crop yield, and reduce environmental pollution
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[0033] Example 1: Study on the role of rhizobium on stem matrix
[0034] 1, seed processing and bacterial liquid configuration
[0035] The stem tumor seeds were selected from the same granules, and the centrifuge tube was placed separately, and the centrifuge is placed in the ultra-clean counter, and the seeds were soaked with 50 min NaClo, and the oscillating centrifuge tube was soaked to ensure that all seeds were soaked. Use the pipette to absorb the sterile water rinse and perform surface disinfection. The flushing liquid is replaced with a pipette, and the gun is 1 ml sterilized gun. The seed disinfected seed is placed in a group culture bottle, and a high-temperature sterilized agar-water solid medium (hereinafter referred to as the medium) surface is poured into the surface of the group culture. Placed in conventional cultures.
[0036]Activity and expansion: Ty medium in a small glass bottle, adding Rhizobium in TY medium, sealing the membrane to the TY medium, sealing th...
Example Embodiment
[0041] Example Different concentrations of rhizobium in Infected Stem Tumoria Inchance
[0042] The surface is sterilized in the surface of the femur, and the sterilization method is placed on the agar-water solid medium. When the seed germination until the two leaves, the forceps eliminate the unculfrated seeds, and the growth is approving the same seedlings. A 50 ml of a centrifugal tube was placed for a few long-term roots, 4500 r / m was centrifuged for 10 min to collect the bacteria, and the PBS buffer was diluted to 1.0 × 10, respectively. 9 CFU / ml, 1.0 × 10 8 CFU / ml, 1.0 × 10 7 CFU / ml, 1.0 × 10 6 CFU / ml four different concentrations of spare. Different concentrations of 1 ml of Rhizobacterial solution were drawn to the medium surface, and 4 repetitions were set each concentration, 10 capsules per bottle, parallel to the equivalent PBS, 4 repetitions. After 9 days, after 9 days, the tumor chip seedlings were investigated and the roulating length and strain height wer...
Example Embodiment
[0046] Example Trimperagus observation
[0047] Under indoor conditions, the full seed surface is disinfected, and the sterilization method is the same. Placed in agar-water solid medium on the culture, germination to the two leaves, each group of inclosures 1ml1.0 × 10 8 CFU / ML PBS diluted bacteria was added to the agar-water solid medium surface, and the stem canide seedlings were used. After bacillia, sealing the membrane seal group culture cylinders to avoid pollution. Place a routine intelligent culture frame rate. The time gradient is provided in the secondary, 6d, 9d, 12d, 15d, 18d, 21d, regularly detected the renapacity of the Stem Majinodans of Stem Majinodans with fluorescence microscopy.
[0048] Picking with representative stem tumors seedlings under each time, 3-4% agar glycid solution was prepared in 100 mL of the triangular bottle, heating agar grease in the microwave oven, melting agarose poured into diameter 2 cm In the centrifuge tube, the slice material is emb...
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