Application of sesamin in the preparation of products for treating and/or preventing lymphoma
A technology of sesamin and lymphoma, applied in the field of lymphoma treatment, can solve the problem of little research on lymphoma, and achieve the effects of inhibiting lymphoma cell proliferation, promoting apoptosis and pyroptosis, and expanding pharmacological effects
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Embodiment 1
[0037] 5×10 5 A mouse T-cell lymphoma model was established by subcutaneous injection of EL4 cells into the right axilla of 6-8 week old BALB / c female mice. When the average tumor volume is about 100mm 3 , tumor-bearing mice were randomly divided into two groups for follow-up treatment. Mice in the treatment group were injected intraperitoneally with 10 mg / kg sesamin every 2 days, diluted with sterile phosphate buffered saline (PBS). Control group (NC) mice were intraperitoneally injected with the same volume of PBS solution. All mice were housed under specific pathogen-free (SPF) conditions with free access to water and food. Tumor volumes were measured every two days. After 21 days, mice were sacrificed, and tumor tissue was collected and weighed. The result is as figure 1 shown. The tumor volume of the mice in the sesamin treatment group was significantly smaller than that of the mice in the control group from about 11 days after tumor inoculation ( figure 1 A); at ...
Embodiment 2
[0039] The cell viability was determined by the CCK-8 method, and the specific method was as follows: 100 μL of cell suspension was prepared in a 96-well plate, with 2000 EL4 cells per well. The culture plate was pre-incubated for 24 h in an incubator (37 °C, 5% CO 2 ). After 24 h, the mice were stimulated with 10 μM, 20 μM and 40 μM sesamin (dissolved sesamin in dimethyl sulfoxide (DMSO), and diluted sesamin with RPMI-1640 medium to a final concentration of 10 μM, 20 μM and 50 μM according to the experimental needs) The mouse T-cell lymphoma cell line EL4 cells (purchased from ATCC, USA) 48, 72 and 96 h, EL4 cells were cultured in RPMI- 1640 medium at 37°C, 5% CO 2 cultured in a humidified incubator. After the specified time, 10 μL of CCK8 solution was added to each well. Incubate the culture plate in an incubator for 1-4 hours, measure the absorbance at 450 nm with a microplate reader, and calculate the cell viability according to the absorbance. If the OD value is not ...
Embodiment 3
[0045] First, EL4 cells were treated with 10 μM, 20 μM and 40 μM sesamin for 72 h, the total protein of each group was extracted, and the protein expression levels of apoptosis-related markers in each group were detected by western blot method, including Bax, Bcl-2 and Caspase-3. It is β-Tubulin, and the specific method is the same as that in Example 2, except that the primary antibodies are Caspase-3 (1:1000), Bax (1:1000), Bcl-2 (1:1000), β-Tubulin (1:3000) ), the secondary antibody is the corresponding secondary antibody.
[0046] The result is as image 3 A and image 3 As shown in B, compared with the control group, the protein expression levels of apoptosis markers (Bax / Bcl-2 and cleaved-Caspase3) in different doses of sesamin-treated groups were significantly increased.
[0047] Second, EL4 cells were treated with 10 μM, 20 μM and 40 μM sesamin for 72 h, and the apoptosis ratio (Annexin V+PI- and Annexin V+PI+) of EL4 cells treated with sesamin was detected by flow cy...
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