Preparation method of high-purity astaxanthin ester from haematococcus pluvialis

A technology of Haematococcus pluvialis and astaxanthin ester, which is applied in the field of preparation of high-purity astaxanthin ester, can solve the problems of complex method, inconvenient industrial production and the like, achieves simple process, high yield, overcomes the problem of astaxanthin The effect of loss

Inactive Publication Date: 2021-07-30
YUNNAN AIERKANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is relatively complicated, and the solvent acetone used is a controlled reagent, and it is not convenient for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Collected Haematococcus pluvialis fresh algae 1000Kg, mass percentage of astaxanthin 3.56%, let it settle down, drain the supernatant, then carry out high-pressure homogeneous wall breaking, the wall breaking pressure is 90MPa, and the wall breaking rate is 94.7%; Add 5wt% lipase to the algae pulp for enzymolysis, control the enzymolysis temperature to 40°C, enzymolysis time to 18 hours, enzymolysis pH value to 6.0, and filter after the enzymolysis is completed. Then the filter cake is extracted with ethanol with a mass concentration of 95%, and after concentration, 0.3kg of astaxanthin ester crude extract is obtained, wherein the mass percentage of astaxanthin is 16.16%; , then add 0.9kg of chromatographic silica gel for adsorption, and dry at 40°C for 1 hour to obtain chromatographic silica gel loaded with astaxanthin ester; pack the chromatographic silica gel loaded with astaxanthin ester into a column, and use a volume ratio of 9:1 in turn , 8:2, 6:4, 4:6 petroleum ...

Embodiment 2

[0030] Collected Haematococcus pluvialis fresh algae 1000Kg, astaxanthin mass percentage 3.56%, let stand to settle, discharge supernatant, then carry out high-pressure homogeneous wall breaking, wall breaking pressure is 90MPa, wall breaking rate is 96.4%; Add 5wt% lipase to the algae pulp for enzymolysis, control the enzymolysis temperature to 40°C, enzymolysis time to 20 hours, enzymolysis pH value to 6.0, and filter after enzymolysis. Then the filter cake is extracted with ethanol with a mass concentration of 95%, and after concentration, 0.27kg of astaxanthin ester crude extract is obtained, wherein the mass percentage of astaxanthin is 17.74%; , then add 0.81kg of neutral alumina for adsorption, and dry at 40°C for 1.5h to obtain neutral alumina loaded with astaxanthin ester; pack the neutral alumina loaded with astaxanthin ester into a column, and use the volume ratio Gradient elution was carried out for 9:1, 8:2, 6:4, 4:6 petroleum ether / ethyl acetate mixture, and then...

Embodiment 3

[0032]Collected Haematococcus pluvialis fresh algae 1000Kg, mass percentage of astaxanthin 3.56%, let stand to settle, discharge supernatant, then carry out high-pressure homogeneous wall breaking, wall breaking pressure is 90MPa, wall breaking rate is 99.0%; Add 5wt% lipase to the algae pulp for enzymolysis, control the enzymolysis temperature to 40°C, enzymolysis time to 24 hours, enzymolysis pH value to 6.0, and filter after the enzymolysis is completed. Then the filter cake is extracted with ethanol with a mass concentration of 95%, and after concentration, 0.16kg of astaxanthin ester crude extract is obtained, wherein the mass percentage of astaxanthin is 27.17%; , then add 0.80kg of neutral alumina for adsorption, and dry at 40°C for 1 hour to obtain neutral alumina loaded with astaxanthin ester; pack the neutral alumina loaded with astaxanthin ester into a column, and use a volume ratio of 9:1, 8:2, 6:4, 4:6 petroleum ether / ethyl acetate mixtures were used for gradient ...

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Abstract

The invention provides a preparation method of high-purity astaxanthin ester from haematococcus pluvialis, and relates to the technical field of astaxanthin ester processing. The preparation method disclosed by the invention comprises the following steps: carrying out homogenization wall-breaking treatment on fresh haematococcus pluvialis, and then adding lipase for enzymolysis; and heating and extracting the enzymolysis product by adopting an ethanol solution, concentrating an extracting solution, conducting dissolving by adopting a weak polar solvent, conducting adsorbing by adopting an adsorbent, conducting drying, conducting packing, adopting petroleum ether / ethyl acetate mixed liquor with different volume ratios as an eluent, carrying out gradient elution, collecting the eluent in sections, and conducting concentrating to respectively obtain the high-purity astaxanthin ester. The method is easy to operate, astaxanthin ester in haematococcus pluvialis can be efficiently extracted, and the obtained astaxanthin ester is high in purity and has high economic value.

Description

technical field [0001] The invention relates to the technical field of astaxanthin ester processing, in particular to a method for preparing high-purity astaxanthin ester derived from Haematococcus pluvialis. Background technique [0002] Astaxanthin has strong antioxidant and biological activity. Yajima et al. studied the effect of astaxanthin on H 2 o 2 Antioxidative and anti-inflammatory effects of induced oxidative stress in human submandibular gland cells, found that 10 μmol / L astaxanthin can significantly reduce the levels of 8-hydroxydeoxyguanosine and interleukin-6 and 8, and reduce the oxidative stress of salivary gland cells impediment. Ying's experiments confirmed that astaxanthin can provide neuroprotection and inhibit the pathological behavior of diabetes induced by inflammation. Zhang et al. studied the promotion of astaxanthin on the apoptosis of myofibroblasts in vivo and in vitro. The experimental results showed that astaxanthin can promote the apoptosis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24C12P23/00
CPCC07C403/24C12P23/00C07C2601/16
Inventor 沈家会李顺友常茹芸王登亮蒋会林王兴勇张勇
Owner YUNNAN AIERKANG BIOTECH
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