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Method for extracting and determining chlorogenic acid in fruit juice by using novel magnetic imprinting material

A technology of imprinted materials and determination methods, which is applied in the direction of material separation, analytical materials, and measuring devices, can solve problems such as difficulties in the accurate determination of target analytes, low content of active ingredients in chlorogenic acid, and complex natural product systems, and achieve rapid power The effect of learning binding rate, good reusability, and significant selectivity

Pending Publication Date: 2021-07-30
FRUIT TREE INST OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the natural product system is complex, macromolecules and small molecules, active and inactive components coexist, there are many structural analogues with similar chemical activities, and the content of active components of chlorogenic acid is low, and it is difficult to extract and measure by general separation methods , which brings difficulties to sample pretreatment and accurate determination of target analytes

Method used

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  • Method for extracting and determining chlorogenic acid in fruit juice by using novel magnetic imprinting material
  • Method for extracting and determining chlorogenic acid in fruit juice by using novel magnetic imprinting material
  • Method for extracting and determining chlorogenic acid in fruit juice by using novel magnetic imprinting material

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Experimental program
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Effect test

Embodiment 1

[0043] Such as Figure 1-12 :

[0044] A novel magnetic imprinting material is used for the extraction and determination method of chlorogenic acid in fruit juice, comprising the following steps:

[0045] S1: Prepare:

[0046] Before carrying out the S2 step, prepare nitrogen (N 2 ), high-purity water, ammonia solution (28%), magnet, absolute ethanol;

[0047] S2: Synthesis:

[0048] In nitrogen (N 2 ) protection, the FeCl 2 4H 2 O and FeCl 3 ·6H 2 O was added to 280 ml of high-purity water, and then stirred, FeCl 2 4H 2 O content is 6.88 g, FeCl 3 ·6H 2 The O content is 18.88 grams, when the stirring temperature reaches the set value, add dropwise in 40 milliliters of ammonia solution to the solution, and the reaction is stirred and reacted for 1 hour at 70°C;

[0049] S3: separation, washing:

[0050] The magnetic solid obtained from step S2 was separated from the reaction mixture using a magnet to obtain black Fe 3 o 4 nanoparticles, then Fe 3 o 4 Nanopart...

Embodiment 2

[0062] Such as Figure 1-12 :

[0063] A novel magnetic imprinting material is used for the extraction and determination method of chlorogenic acid in fruit juice, comprising the following steps:

[0064] S1: Prepare:

[0065] Before carrying out the S2 step, prepare nitrogen (N 2 ), high-purity water, ammonia solution (28%), magnet, absolute ethanol;

[0066] S2: Synthesis:

[0067] In nitrogen (N 2 ) protection, the FeCl 2 4H 2 O and FeCl 3 ·6H 2 O was added to 280 ml of high-purity water, and then stirred, FeCl 2 4H 2 O content is 6.88 g, FeCl 3 ·6H 2 The O content is 18.88 grams, when the stirring temperature reaches the set value, add dropwise in 40 milliliters of ammonia solution to the solution, and the reaction is stirred and reacted for 1 hour at 70°C;

[0068] S3: separation, washing:

[0069] The magnetic solid obtained from step S2 was separated from the reaction mixture using a magnet to obtain black Fe 3 o 4 nanoparticles, then Fe 3 o 4 Nanopart...

Embodiment 3

[0081] Such as Figure 1-12 :

[0082] A novel magnetic imprinting material is used for the extraction and determination method of chlorogenic acid in fruit juice, comprising the following steps:

[0083] S1: Prepare:

[0084] Before carrying out the S2 step, prepare nitrogen (N 2 ), high-purity water, ammonia solution (28%), magnet, absolute ethanol;

[0085] S2: Synthesis:

[0086] In nitrogen (N 2 ) protection, the FeCl 2 4H 2 O and FeCl 3 ·6H 2 O was added to 280 ml of high-purity water, and then stirred, FeCl 2 4H 2 O content is 6.88 g, FeCl 3 ·6H 2 The O content is 18.88 grams, when the stirring temperature reaches the set value, add dropwise in 40 milliliters of ammonia solution to the solution, and the reaction is stirred and reacted for 1 hour at 70°C;

[0087] S3: separation, washing:

[0088] The magnetic solid obtained from step S2 was separated from the reaction mixture using a magnet to obtain black Fe 3 o 4 nanoparticles, then Fe 3 o 4 Nanopart...

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Abstract

The invention relates to the technical field of chlorogenic acid extraction and discloses a method for extracting and measuring chlorogenic acid in fruit juice by using a novel magnetic imprinting material. The method comprises the following steps of S1, preparation, before the step S2 is carried out, firstly preparing nitrogen (N2), high-purity water, an ammonia water solution (28%), a magnet and absolute ethyl alcohol; S2, synthesizing, under the protection of nitrogen (N2), ultrasonically dispersing FeCl2. 4H2O and FeCl3. 6H2O into 280ml of the high-purity water; on the basis of a method for extracting and measuring chlorogenic acid in fruit juice based on a novel magnetic imprinting material, the synthesized Fe3O4-coated MIPs are shorter in chlorogenic acid absorption equilibrium time and larger in saturated absorption capacity, and compared with structural analogues of chlorogenic acid, the Fe3O4-coated MIPs are better in chlorogenic acid selectivity, higher in anti-interference capability in actual fruit juice sample extraction and higher in detection sensitivity; the synthesized Fe3O4-coated MIPs have high adsorption capacity, high dynamic binding rate and remarkable selectivity on chlorogenic acid (CGA), and can be successfully applied to extraction and determination of chlorogenic acid in an actual sample.

Description

technical field [0001] The invention relates to the technical field of chlorogenic acid extraction, in particular to a method for extracting and measuring chlorogenic acid in fruit juice using a novel magnetic imprinting material. Background technique [0002] Molecular imprinting technology (MIT) is a new and efficient molecular recognition technology developed based on the principle of antigen-antibody specific recognition. In recent years, the use of Magnetic Molecularly Imprinted Polymers (MMIPs) in sample pretreatment has attracted more and more attention from researchers. Due to the addition of magnetic media, MMIPs can be rapidly separated from the matrix under the action of an external magnetic field without centrifugation or filtration, so they are widely used in food testing, environmental analysis, and biological analysis. [0003] Chlorogenic acid, a phenolic acid widely distributed in fruits, vegetables, plants, it has anti-tumor, anti-oxidant, anti-inflammator...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8631G01N2030/065
Inventor 程杨徐国锋李海飞李静闫震李银萍匡立学沈友明张建一高贯威佟瑶刘红弟
Owner FRUIT TREE INST OF CHINESE ACAD OF AGRI SCI
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