A kind of preparation method of polymer vesicles
A technology for polymers and vesicles is applied in the field of the preparation of polymer vesicles, the composite process of thin film annealing technology and hydration method to prepare polymer vesicles, and can solve the problems of unfavorable cell delivery and slow formation of vesicles.
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Embodiment 1
[0032] Weigh 20mg of block copolymer PEG 5000 -PLA 12000 , added to 2 mL of THF, and stirred (500 r / min) for 15 minutes to obtain a block copolymer solution of 10 mg / mL; the block copolymer solution was evaporated to remove THF to obtain a transparent film; placed at 80 °C for vacuum drying In the box, the film was thermally annealed for 12 h. Then, 2 mL of deionized water was added, and the polymer vesicles were prepared by stirring at room temperature (400 r / min) for 12 h. The transmission electron microscope image of the polymer vesicles prepared in this example is as follows image 3 As shown in (1), the results show that uniform nano-scale vesicles can be formed under short-time stirring with the technology of the present invention (film annealing + hydration method), while the traditional technology (ordinary film hydration method, no Thin film thermal annealing operation) at the same time, the number of vesicles generated is less, and irregular aggregates appear ( ...
Embodiment 2
[0034] Weigh 20mg of block copolymer PEG 2000 -PCL 7500 , added to 2 mL of THF, and stirred (100 r / min) for 30 minutes to obtain a block copolymer solution of 5 mg / mL; the block copolymer solution was blown dry with nitrogen to remove THF to obtain a transparent film; placed in a vacuum at 70 °C In a drying oven, the films were thermally annealed for 10 h. Then, 2 mL of deionized water was added, and the polymer vesicles were prepared by stirring at room temperature (400 r / min) for 18 h.
Embodiment 3
[0036] Weigh 20mg of block copolymer PEG 4000 -PLA 20000 -PEG 4000 , added to 2 mL of THF, and stirred (500 r / min) for 15 minutes to obtain a block copolymer solution of 10 mg / mL; the block copolymer solution was evaporated to remove THF to obtain a transparent film; placed at 80 °C for vacuum drying In the box, the film was thermally annealed for 12 h. Then, 2 mL of deionized water was added, and the polymer vesicles were prepared by stirring at room temperature (400 r / min) for 24 h.
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Abstract
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