Micro-fluidic chip for extracting nucleic acid and preparation method and using method thereof

Abstract

The invention relates to the technical field of medical instruments, in particular to a micro-fluidic chip for extracting nucleic acid and a preparation method and using method thereof. The micro-fluidic chip comprises a chip body, an extraction channel and a sample channel are formed in the chip body, a front liquid guide hole is formed in one end of the extraction channel, and the other end of the extraction channel is communicated with the sample channel; an extraction hole is formed in the extraction channel close to the front liquid guide hole; a trailing liquid hole is formed in one end of the sample channel, and a sample adding hole is formed in the position, close to the trailing liquid hole, of the sample channel; the iron wax valve is arranged at the communication position of the extraction channel and the sample channel, and exhaust holes are formed in the positions, close to the iron wax valve, of the extraction channel and the sample channel respectively; the trailing liquid hole is connected with the anode of power equipment; the front liquid guide hole is connected with the cathode of the power equipment. According to the method, nucleic acid is extracted and concentrated on the micro-fluidic chip in a constant-speed electrophoresis mode, the steps are simple, the nucleic acid is not prone to being polluted, and high-purity nucleic acid is obtained within a relatively short time.

Description

technical field [0001] The invention relates to the technical field of medical devices, in particular to a microfluidic chip for extracting nucleic acid, a preparation method and a use method thereof. Background technique [0002] Nucleic acid is one of the basic substances of life, and nucleic acid extraction is a necessary step for nucleic acid detection. Common nucleic acid extraction and purification methods include liquid phase extraction technology such as phenol-chloroform extraction technology and solid phase extraction methods, including spin column method and magnetic bead method. The solid-phase extraction method needs to add chaotropic salts during the lysis process to destabilize proteins (including nucleases) and dissociate nucleic acids, so that nucleic acids can bind to the surface of silica gel columns or magnetic beads, and then use buffers to remove unresolved nucleic acids. bound molecules, and finally a solvent is used to release the bound nucleic acid ...

AI Technical Summary

Problems solved by technology

[0003] Therefore, the technical problem to be solved by the present invention is to overcome the cumbersome and time-consuming defects of the method for extracting nucleic acid in the prior art, thereby providing a microfluidic chip for extracting nucleic acid and its preparation method and use method

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