Tissue culture propagation method of endangered rare plant tetraena mongolica

Abstract

The invention belongs to the technical field of plant asexual propagation, and discloses a tissue culture propagation method of an endangered rare plant tetraena mongolica. Tissue culture is intensively embodied in collection and pretreatment of an explant of the tetraaceae mongolian and the completion part of each treatment stage of the tetraaceae mongolian. A tissue culture technology is applied, and annual tetraena mongolica plants are directly induced to root for propagation. The annual new branches are taken as explants, and washing with running water is conducted to remove attachments such as dust and the like on the surfaces of the explants; and a cleaning agent is adopted to disinfect and sterilize the surface of the explants. The sterilizing agent is used for disinfecting and sterilizing the explants, the sterile environment of the treated explants is guaranteed through secondary flushing of sterile water, and the sterilizing agent is matched with the optimal culture medium to complete tissue culture of the tetraena mongolica. According to the culture mode, the technical bottleneck of artificial breeding and cultivation of the tetraena mongolica is broken through, and artificial rapid breeding and large-scale production and application of endangered desert plants are achieved. A large number of tetraena mongolica plants are obtained through a tissue culture asexual propagation means, artificial propagation of tetraena mongolica is achieved, and basic conditions are provided for continuation and expansion of populations.

Description

technical field [0001] The invention belongs to the technical field of plant asexual reproduction, and in particular relates to a method for tissue culture and propagation of endangered and rare plant quadrangleia. Background technique [0002] Sihemu is a unique single-species plant in China, and it is also the most representative ancient relic, rare and endangered plant on the earth, and is known as "the living fossil of plants". Sihemu is a first-class protected plant in Inner Mongolia. It is designated as a second-class national protected plant in the list of national key protected wild plants (the first batch), and it is also identified as a national first-class plant in the list of national key protected wild plants (second batch). Protected plants, known as "giant pandas in plants", are now only dotted and distributed in some areas of the grassland desert between the Inner Mongolia Autonomous Region and Shizuishan in eastern Ningxia, and they grow concentratedly in In...

AI Technical Summary

Problems solved by technology

Tetraphyllum has two types of styles, one type is that the style extends out of the flower before flowering, and the stamens also extend out of the corolla during flowering, but the stamens and pistils are different in length, and the stigma is higher than the anther; the other type is the style before flowering Does not elongate until the style is always lower than the position of the anthers during flowering, which is not conducive to pollination

[0006] Moreover, the seeds of tetrahex wood have high oil content, and many insects and small animals like to eat them, so that a small amount of plump seeds lose the ability to germinate due to being eaten by moths, which will cause the loss of part of the seeds

The seed coat of the tetrahexa seeds will inhibit the germination of the seeds, and the quadranglewood seeds have no post-ripening and vernalization effects. The solid seeds of the year will start to germinate after they fall to the ground. It is difficult for the seedlings to survive in a harsh environment, and it is found that The secretions of the quadrangle wood itself have an impact on its seed germination and seedling survival, and will cause the roots of the seedlings to rot

[0008] It is difficult for the existing asexual propagation techniques commonly used in quadrangle wood to achieve fast and efficient, get rid of the constraints of natural geographical environment and seasons

At present, there are reports on the propagation method of quadrangle wood with cuttings, but the propagation coefficient of cuttings is low and affected by seasons and regions

Tissue culture generally adopts the method of inducing callus to reproduce, but related studies have found that after the callus is transferred many times, there will be chromosomal variation

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