Pig blood deproteinized extract gel and preparation method thereof

A deproteinization and extract technology, applied in the directions of drug combination, pharmaceutical formulation, aerosol delivery, etc., can solve the problems of wound healing effect, easy water loss, poor anti-infection and anti-exudation ability, etc. Injury, inhibit cell loss, compensate for potential risk effects

Active Publication Date: 2012-01-04
沈阳衡天然生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many types of medicines and treatment methods for the treatment of diseases in this area. For large area I o -Ⅲ o The treatment of burns is exposure therapy, that is, treatment with lamp roasting or infrared radiation to reduce exudation and anti-infection. For small area burns, most of them use ointment, tincture, etc. to apply exposure or semi-exposure treatment. The shortcomings of these methods are anti-infection and Poor anti-exudation ability, long course of treatment, great pain, high scarring rate, high cost, especially the psychological disorder caused by long-term exposure to nakedness, which seriously affects sleep and appetite, and has a certain impact on wound healing
[0003] For ocular surface inflammation, antibiotics are generally used in combination with vitamins and trace hormones (including eye drops) for treatment. This treatment effect is not ideal, especially for acute inflammatory lesions, and for prolonged unhealed ocular surface ulcers (alkali burns) Ulcers, radiation ulcers and neurotrophic ulcers) and infectious ulcers have no satisfactory treatment, often causing loss of vision, resulting in permanent disability of the patient
[0004] At present, the drugs of the same kind to treat the above-mentioned wounds, burns, scalds and corneal injuries all use calf blood as the raw material to remove the protein extract. Sex and other factors, the World Health Organization strictly restricts the use of bovine blood raw material products, and the ge

Method used

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  • Pig blood deproteinized extract gel and preparation method thereof
  • Pig blood deproteinized extract gel and preparation method thereof
  • Pig blood deproteinized extract gel and preparation method thereof

Examples

Experimental program
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Embodiment 1

[0024] Preparation of pig blood deproteinized extract solution: According to the preparation method described in the patent No. ZL200510046289.1, the title of the invention is "The method of extracting active substances that stimulate cell respiration from pig blood": take the healthy pigs that have passed the inspection in the non-infected area, use aseptic blood collection, and remove Fibrin, 40L of defibrinated blood was obtained, repeated freezing and thawing (-20℃~40℃) 2-4 times; homogenized in a colloid mill at 2-8℃ for 2 minutes, repeated 3 times; take the homogenate in After centrifuging at 4000 rpm for 20 minutes, discard the precipitate; take the supernatant, and ultrafilter it with hollow fiber ultrafiltration columns with a molecular weight cut-off of 100000D, 30000D, 10000D, and 6000D respectively to obtain 28.8 L of filtrate D; Concentrate under reduced pressure at low temperature (25°C) (to a total solid content above 40 mg / ml) to obtain 4800 g of pig blood dep...

Embodiment 2

[0026] Preparation of porcine blood deproteinized extract gel: This example contains 20% of pig blood protein-free extract, and 1000 pig blood protein-free extract gels are made.

[0027] Take a 10L container, take 100g of methylcellulose, 500g of glycerin, stir with a glass rod, disperse evenly, add 2000g of water for injection after fully wetting, add 10g of methyl ethyl paraben, stir evenly, add 0.1mol / L sodium hydroxide Appropriate amount, adjust the pH value to 7.5-8.0, autoclave at 115°C for 30 minutes, take it out, cool it, and get the matrix for later use; add 1000g of pig blood deproteinized extract solution to the matrix under sterile conditions, and then add water for injection to a total weight of 5000g , adjust the pH to 6.0-8.0 with 0.1mol / L sodium hydroxide or hydrochloric acid, mix well to form a gel, stir evenly with a sterilized glass rod, and aseptically dispense, 5g / bottle, to obtain a total of 1000 pig blood Deproteinized extract gel.

Embodiment 3

[0029] Preparation of porcine blood deproteinized extract gel: This example contains 27.5% of pig blood protein-free extract, and 1000 pig blood protein-free extract gels are made.

[0030]Take a 10L container, take 150g of methylcellulose, 562.5g of glycerin, stir with a glass rod, disperse evenly, add 1750g of water for injection after fully wetting, add 13.75g of methyl ethyl paraben, stir evenly, add 0.1mol / L hydrogen Appropriate amount of sodium oxide, adjust the pH value to 7.5-8.0, autoclave at 115°C for 30 minutes, take it out, cool it, and obtain the matrix for later use; add 1375 g of pig blood deproteinized extract solution to the matrix under aseptic conditions, and then add water for injection to the total Weigh 5000g, adjust the pH to 6.0-8.0 with 0.1mol / L sodium hydroxide or hydrochloric acid, mix well to form a gel, stir evenly with a sterilized glass rod, pack aseptically, 5g / bottle, a total of 1000 pieces Pig blood deproteinized extract gel.

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Abstract

The invention discloses a pig blood deproteinized extract preparation, particularly relates to pig blood deproteinized extract gel, more particularly relates to a medicament for treating skin and eye inflammatory injuries caused by various reasons and a preparation method thereof, and belongs to the technical field of biological pharmacy, wherein the medicament is a novel preparation of a pig blood deproteinized extract. The pig blood deproteinized extract gel is prepared from the following raw material drugs in parts by weight: 20 to 50 parts of pig blood deproteinized extract solution, 2 to6 parts of methylcellulose, 10 to 15 parts of glycerol, and 0.2 to 0.5 part of methyl and ethyl hydroxybenzoate. Compared with similar bovine blood products, the medicament made from pig blood has the product advantages of high safety and high bioactivity. The pig blood deproteinized extract gel makes up potential hazards of bovine blood products, and the safety and curative effect of the pig blood deproteinized extract gel are remarkably higher than those of calf blood deproteinized gel.

Description

technical field [0001] The present invention relates to a porcine blood deproteinized extract preparation, in particular to a porcine blood deproteinized extract gel, and more specifically, to a medicine for treating skin and eye inflammation damage caused by various reasons and a preparation method thereof , which is a new dosage form of pig blood deproteinized extract, belonging to the technical field of biopharmaceuticals. Background technique [0002] Surgical wounds, burns, scalds, and diseases including bedsores, limb and skin ulcers are very common skin diseases. At present, there are many types of medicines and treatment methods for the treatment of diseases in this area. For large area I o -Ⅲ o The treatment of burns is exposure therapy, that is, treatment with lamp roasting or infrared radiation to reduce exudation and anti-infection. For small area burns, most of them use ointment, tincture, etc. to apply exposure or semi-exposure treatment. The shortcomings of ...

Claims

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Application Information

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IPC IPC(8): A61K35/14A61K47/38A61K9/06A61P17/00A61P27/02
Inventor 王光李莉万琳琳刘路刘延斌
Owner 沈阳衡天然生物科技有限公司
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