PEEK composite biological implant material loaded with BMP-2 gene slow-release coating and preparation method of PEEK composite biological implant material

A BMP-2, implant material technology, applied in coating, tissue regeneration, drug delivery, etc., can solve the problems of difficult to obtain osseointegration effect, poor biological activity, low surface energy, etc., to promote calcium ion deposition , the effect of inducing osteogenic differentiation of cells and increasing the surface area

Active Publication Date: 2021-08-06
JILIN UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, PEEK is a biologically inert material. Its hydrophobic surface and high reactive inertia lead to low surface energy

Method used

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  • PEEK composite biological implant material loaded with BMP-2 gene slow-release coating and preparation method of PEEK composite biological implant material
  • PEEK composite biological implant material loaded with BMP-2 gene slow-release coating and preparation method of PEEK composite biological implant material
  • PEEK composite biological implant material loaded with BMP-2 gene slow-release coating and preparation method of PEEK composite biological implant material

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Embodiment 1

[0047] 1) Dissolve 5 g of PLGA containing a free carboxyl terminal (the molar ratio of lactic acid and glycolic acid monomers is 50:50, Mw=10000) in 50 mL of dichloromethane, and then add 45.4 mg of dicyclohexylcarbodiimide and 25.3mg of N-succinimide, after stirring for 12h, the reaction product was separated and precipitated in anhydrous ether, and vacuum-dried to obtain carboxyl-activated PLGA;

[0048] 2) Add 375 mg of hydrazine hexaethylene glycol and the carboxyl-activated PLGA obtained in step 1) into 50 mL of anhydrous DMSO, stir for 12 hours, then add the reaction product to 4°C absolute ethanol, separate the precipitate and dry it in vacuo to obtain Aminated PLGA sustained-release microspheres, denoted as PLGA-NH 2 Slow-release microspheres;

[0049] 3) pCMV6-XL4 was digested with Not I, treated with T4 DNA polymerase, and then digested with Eco RI to obtain a linear DNA fragment containing the BMP-2 target gene, which had both a sticky end and a blunt end; The typ...

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Abstract

The invention discloses a PEEK composite biological implant material loaded with a BMP-2 gene slow-release coating and a preparation method of the PEEK composite biological implant material, and belongs to the technical field of biomedical materials. The preparation method comprises the following steps: preparing PLGA-NH2 sustained-release microspheres loaded with the BMP-2 gene by utilizing a multiple emulsion solvent evaporation method; meanwhile, preparing PEEK with nano topological morphology and a nitrogen-containing functional group by adopting a nitrogen low-temperature plasma radiation technology, constructing a PDA bionic coating on the surface of the PEEK by virtue of the'wet bonding characteristic 'of PDA, and PDA is used as a secondary reaction platform to graft PLGA-NH2 sustained-release microspheres loaded with the BMP-2 gene, so that a controlled-release gene sustained-release system is constructed on the surface of the PEEK and the composite biological implant material disclosed by the invention is obtained. The PLGA microspheres are introduced as a sustained-release coating, so that the effective release time of bone growth factors is prolonged, the effective release time is matched with the key period of bone defect healing, a stable and long-term bone formation process is realized, and the drug loading rate and the entrapment rate are relatively high.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a polyether ether ketone (PEEK) composite biological implant material loaded with bone morphogenetic protein-2 (BMP-2) gene slow-release coating and a preparation method thereof. Background technique [0002] As a high-performance implant material, polyetheretherketone (PEEK) and its composite materials have good biocompatibility, high strength, high hardness, corrosion resistance, wear resistance, high temperature resistance and elastic modulus with bone tissue. It is one of the non-metallic bioprosthetic materials with wide application prospects. PEEK composite materials have been used as orthopedic implants in the fields of spinal surgery, joint surgery, and trauma repair, and have achieved good repair results. However, PEEK is a biologically inert material. Its hydrophobic surface and high reactivity lead to low surface energy and poor biological act...

Claims

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Application Information

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IPC IPC(8): A61L27/34A61L27/18A61L27/54
CPCA61L27/18A61L27/34A61L27/54A61L2400/18A61L2430/02A61L2420/02A61L2420/06A61L2300/258A61L2300/622A61L2300/602C08L61/16C08L67/04C08L79/02
Inventor 刘红秦爽姜玲玲路政宽陈天杰刘称称高蕴波
Owner JILIN UNIV
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