Manufacturing method of edible chitosan/sodium alginate/gelatin 3D scaffold for cell culture meat

A technology of sodium alginate and cell culture, which is applied in the direction of cell culture support/coating, animal cells, tissue culture, etc. It can solve the problems of high R&D cost of cell culture meat and the inability to proliferate cells in large quantities, and achieve cost reduction and good quality. The effect of simple cell adhesion and gelation conditions

Inactive Publication Date: 2021-08-06
NORTHWEST A & F UNIV
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Problems solved by technology

[0007] The purpose of the present invention is to provide a method for manufacturing an edible chitosan/sodium alginate/gelatin 3D scaffold for cell cult

Method used

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  • Manufacturing method of edible chitosan/sodium alginate/gelatin 3D scaffold for cell culture meat
  • Manufacturing method of edible chitosan/sodium alginate/gelatin 3D scaffold for cell culture meat
  • Manufacturing method of edible chitosan/sodium alginate/gelatin 3D scaffold for cell culture meat

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Embodiment Construction

[0022] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0023] 1. Fabrication of chitosan / sodium alginate / gelatin 3D scaffolds:

[0024] (1) Dissolve chitosan, gelatin and collagen in 0.5-3% acetic acid solution to obtain chitosan solution, gelatin solution and collagen with concentrations of 0.5-3%, 0.5-5%, and 0.5-5% respectively solution; dissolving sodium alginate in water to obtain a sodium alginate solution with a concentration of 0.5 to 3%;

[0025] (2) Mix gelatin and collagen solution at a mass ratio of 1:5 to 5:1, and mix chitosan solution with gelatin and collagen solution at a mass ratio of 3:1 to 1:1;

[0026] (3) Mix sodium alginate solution with chitosan, gelatin and collagen mixed solution at a mass ratio of 1:3~3:1;

[0027] (4) The mixed solution was stirred and reacted at room temperature for more than 2 hours, and then freeze-dried to obtain a 3D scaffold.

[0028] 2. Skeleta...

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Abstract

The invention relates to a manufacturing method of an edible chitosan/sodium alginate/gelatin 3D scaffold for cell culture meat. The chitosan/sodium alginate/gelatin 3D scaffold manufactured by the method can be used for culture of skeletal muscle cells and can provide support for manufacturing of the cell culture meat. The invention belongs to the field of future food science and technology. According to the manufacturing method, chitosan with certain concentration, sodium alginate, gelatin and collagen are uniformly mixed according to a certain ratio, reacted at room temperature for more than 2 hours, and freeze-dried to obtain the chitosan/sodium alginate/gelatin 3D scaffold. The manufacturing method is simple and efficient, the 3D scaffold is formed through electrostatic interaction among the chitosan, the gelatin and the sodium alginate under the condition that a toxic cross-linking agent is not used, and the gelatin is used for partially replacing collagen to serve as a substance for promoting cell adhesion, so that the obtained chitosan/sodium alginate/gelatin 3D scaffold has good cell adhesion and compatibility, and can be used for skeletal muscle cell culture; the manufacturing method of the novel and non-toxic 3D scaffold is provided by the invention for the research of the cell culture meat.

Description

technical field [0001] The invention relates to the field of future food science and technology, in particular to a method for manufacturing an edible chitosan / sodium alginate / gelatin 3D scaffold for cell cultured meat. Background technique [0002] Meat is a valuable source of animal protein, however, the current traditional meat production capacity is close to the maximum level, almost two-thirds of the agricultural land is used to raise livestock, and the remaining one-third can barely meet human needs. Demand for plant-based foods. Meat production is one of the major contributors to global environmental degradation. Currently, livestock raised for meat production consumes 30% of the world's ice-free land and 8% of the world's freshwater, while generating 18% of total global greenhouse gas (GHG) emissions. Livestock is also one of the major contributors to deforestation and degradation of wildlife habitat. Globally, 34% of livestock-related greenhouse gas emissions are...

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Application Information

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IPC IPC(8): C12N5/077
CPCC12N5/0658C12N2533/72C12N2533/74C12N2533/54C12N2513/00
Inventor 冯宪超周光宏陈琳陈燕李鳞子刘亚平陈晓红
Owner NORTHWEST A & F UNIV
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