Eosin dye liquor and preparation method thereof

Abstract

The invention discloses eosin dye liquor and a preparation method thereof, the eosin dye liquor comprises the following components in percentage by mass: 99.3-99.5 percent of distilled water, 0.1-0.3 percent of water-soluble eosin Y, 0.05-0.2 percent of glacial acetic acid, 0.01-0.1 percent of dyeing assistant and 0.05-0.1 percent of preservative. The preparation method comprises the following steps: S1, weighing the distilled water, the water-soluble eosin Y, the glacial acetic acid, the dyeing assistant and the preservative according to the ratio; S2, sequentially adding distilled water, water-soluble eosin Y, a dyeing assistant and a preservative into a beaker, and uniformly stirring to obtain a mixed solution; S3, adding glacial acetic acid into the mixed solution, and adjusting the PH value of the mixed solution to 5.5-6.5; and S4, filtering the mixed solution with the adjusted PH value to obtain filtrate, namely eosin dye liquor.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to an eosin dye solution and a preparation method thereof. Background technique [0002] Hematoxylin-eosin staining (hematoxylin-eosinstaining), referred to as HE staining, is one of the commonly used staining methods in paraffin section technology. The hematoxylin staining solution is alkaline, which mainly makes the chromatin in the nucleus and the nucleic acid in the cytoplasm stain purple blue; eosin is an acid dye, which mainly makes the components in the cytoplasm and extracellular matrix red. HE staining is the most basic and widely used technical method in the teaching and scientific research of histology, embryology and pathology. [0003] The operating steps in the hematoxylin-eosin staining method mainly include slicing—baking—dewaxing—hydration—hematoxylin staining—differentiation—bluening—eosin staining—dehydration—transparency—sealing; among them, eosin staining mainly Us...

AI Technical Summary

Problems solved by technology

[0005] Although the existing water-soluble eosin dye formula is simple, but the dyeing time is long and the dye solution has a short validity period, it is often necessary to add glacial acetic acid to cooperate with it, but the dosage of glacial acetic acid is difficult to control, and the addition of a small amount cannot achieve the effect of promoting dyeing Adding too much will cause the dye solution to easily precipitate, and the color tone will be incorrect and easy to fade, and the dyeing result cannot be stored for a long time