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A nanocomposite-based LAPs sensor for the detection of GPC3 for non-diagnostic purposes

A nano-composite material and sensor technology is applied in the field of GPC3 detection based on LAPS sensor, which can solve the problems of expensive kits, cumbersome and complicated methods and operations, and achieve the effects of improving response speed and detection sensitivity, promoting electron transfer, and improving quality transfer.

Active Publication Date: 2022-05-31
GUILIN UNIV OF ELECTRONIC TECH
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Problems solved by technology

The invention patent with the publication number CN112014577A discloses a kit for improving the detection sensitivity of GPC3 and its preparation method; but the method is cumbersome and complicated to operate, and the kit is expensive

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  • A nanocomposite-based LAPs sensor for the detection of GPC3 for non-diagnostic purposes
  • A nanocomposite-based LAPs sensor for the detection of GPC3 for non-diagnostic purposes
  • A nanocomposite-based LAPs sensor for the detection of GPC3 for non-diagnostic purposes

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Embodiment Construction

[0039] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.

[0040] figure 1 Schematic diagram of a LAPS-based sensor for GPC3 detection: H-rGO-Pt@Pd NPs nanocomposites were synthesized by a one-step reduction method; activated with NaOH solution and APTES solution, and glutaraldehyde was used as a coupling agent to separate H-rGO-Pt@Pd NPs. -rGO-Pt@Pd NPs composite nanomaterials and GPC3 Apt Coupling on a LAPS chip to form a LAPS sensitive unit, and then placing the GPC3 solution on the LAPS sensitive unit to obtain a LAPS sensor. Under the action of external bias voltage, due to GPC3 AptThe specific recognition between GPC3 and GPC3 causes the potential change in the LAPS-sensitive cell, resulting in a corresponding shift in the (I-V) curve of the LAPS sensor. The LabVIEW platform was used to record the voltage shift value of the LAPS sensor with different GPC3 concentrations. , so as to realize the...

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Abstract

A method for detecting GPC3 based on a nanocomposite LAPS sensor, designed and synthesized H‑rGO‑Pt@Pd NPs nanocomposites by a one-step reduction method; the LAPS chip was activated with NaOH and APTES, and glutaraldehyde was used as a coupling agent. Composite nanomaterials and GPC3 Apt Coupling with the activated LAPS chip to form a LAPS sensitive unit, and placing the GPC3 solution on the LAPS sensitive unit to obtain an LAPS sensor. Using GPC3 Apt The specific recognition with GPC3 causes the change of the potential in the sensitive unit of LAPS to realize the detection of GPC3, and the minimum detection limit reaches 0.212 ng / mL.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a method for detecting GPC3 based on a LAPS sensor. Background technique [0002] At present, the detection methods of glypican-3 (GPC3) mainly include enzyme-linked immunosorbent assay, radioimmunoassay, electrochemical analysis, and time-resolved fluorescence immunoassay. The invention patent with publication number CN112210018A discloses a chimeric antigen receptor targeting GPC3 and its application; the chimeric antigen receptor includes an antigen binding domain, a hinge region, a transmembrane domain and a signal transduction domain , but the method requires high operation means and technology. The invention patent with publication number CN112014577A discloses a kit for improving GPC3 detection sensitivity and a preparation method thereof; however, the method is complicated and complicated in operation, and the kit is expensive. There is a need t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/537G01N33/543
CPCG01N33/537G01N33/54326G01N33/54353
Inventor 李桂银李文湛冯华富陈伟梁晋涛周治德
Owner GUILIN UNIV OF ELECTRONIC TECH