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Miller tube inhibitor receptor chemiluminescence immunoassay kit and preparation method thereof

A technology of chemiluminescence immunity and detection kits, which is applied in the direction of chemiluminescence/bioluminescence, biological testing, and analysis through chemical reactions of materials, etc. It can solve the problems of few methods, achieve strong anti-interference ability and improve sensitivity and accuracy, reducing the effect of human error

Active Publication Date: 2021-08-10
DIRUI MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Few methods exist to measure Müllerian inhibitor receptors

Method used

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  • Miller tube inhibitor receptor chemiluminescence immunoassay kit and preparation method thereof
  • Miller tube inhibitor receptor chemiluminescence immunoassay kit and preparation method thereof
  • Miller tube inhibitor receptor chemiluminescence immunoassay kit and preparation method thereof

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preparation example Construction

[0050] The preparation method of the Müllerian inhibitor receptor chemiluminescent immunoassay kit of the present invention comprises the following steps:

[0051] Step 1, R1 reagent preparation

[0052] Take the streptavidin carboxyl magnetic particle solution and place it on the magnetic separator until the supernatant is free of turbidity, discard the supernatant, keep the magnetic particles, wash with buffer Ⅰ repeatedly for 3 to 5 times, and use buffer Ⅰ to prepare The solid-phase reagent whose mass percentage concentration of magnetic particles is 0.01%-1%, is the R1 reagent, and is stored at 2-8°C.

[0053] Wherein, the streptavidin carboxyl magnetic particle solution can be purchased from Agilent Technologies Co., Ltd., with a concentration of 10-100 mg / ml.

[0054] Step 2, R2 reagent preparation

[0055] Put the Müllerian inhibitor receptor monoclonal antibody into a centrifuge tube, and centrifuge at room temperature for 20-30s, so that the Müllerian inhibitor rece...

Embodiment 1

[0069] Preparation of R1 reagent: Take 0.5 milliliter (50 mg) of streptavidin carboxyl magnetic particle solution with a concentration of 100 mg / ml, place it on a magnetic separator until the supernatant is free of turbidity, discard the supernatant, and keep the magnetic particles. With 50mM MES, 0.05% Tween-20, the buffer solution that pH is 6.5 washes repeatedly 3 times, in 50mM MES, 0.05% Tween-20, the buffer solution that pH is 6.5 is made up the magnetic bead concentration is 0.072wt% R1 reagent.

[0070] Preparation of R2 reagent: put 500 μg Müllerian inhibitor receptor monoclonal antibody into a centrifuge tube, make sure the antibody is at the bottom of the centrifuge tube, add carbonic acid buffer solution, mix well, add acridinium ester DMF after mixing The solution is blocked and purified to obtain the Müllerian inhibitor receptor monoclonal antibody with a molar ratio of 1:3 between the Müllerian inhibitor receptor monoclonal antibody and the chemiluminescent mark...

Embodiment 2

[0073] Preparation of R1 reagent: Take 0.5 milliliter (50 mg) of streptavidin carboxyl magnetic particle solution with a concentration of 100 mg / ml, place it on a magnetic separator until the supernatant is free of turbidity, discard the supernatant, and keep the magnetic particles. After repeated washing with 50mM MES, 0.05% Tween-20, pH6.5 buffer solution for 3 times, make R1 with a magnetic bead concentration of 0.072% in 50mM MES, 0.05% Tween-20, pH6.5 buffer solution reagent.

[0074] Preparation of R2 reagent: put 500 μg Müllerian inhibitor receptor monoclonal antibody into a centrifuge tube, make sure the antibody is at the bottom of the centrifuge tube, add carbonic acid buffer solution, mix well, add acridinium ester DMF after mixing The solution is sealed and purified to obtain the Müllerian inhibitor receptor monoclonal antibody in which the molar ratio of the Müllerian inhibitor receptor monoclonal antibody to the chemiluminescent marker substance is 1:5. The conc...

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Abstract

The invention relates to a Miller tube inhibitor receptor chemiluminescence immunoassay kit and a preparation method thereof, which belong to the technical field of kits. The kit provided by the invention comprises streptavidin carboxyl magnetic particles, a buffer solution II containing a chemiluminescent marker-labeled Miller tube inhibitor receptor monoclonal antibody, and a coupling marker-labeled Miller tube inhibitor receptor derivative, wherein the buffer solution II comprises 0.01 wt%-0.06 wt% of an additive, and the additive is one or two of poly (aminopropyl) biguanide and polyhexamethylene biguanide. The kit is simple to operate, pollution-free, high in sensitivity and wide in detection range.

Description

technical field [0001] The invention belongs to the technical field of kits, and in particular relates to a Müllerian duct inhibitor receptor chemiluminescent immunoassay kit and a preparation method thereof. Background technique [0002] In terms of assisted reproductive technology, if the ovarian response can be accurately predicted and an individualized stimulation plan can be formulated, the pregnancy rate can be increased to a certain extent and the risk of complications can be reduced. During the transition period from primordial follicles to growing follicles and the early antral follicle stage, Müllerian inhibitory receptor (MISR) directly or indirectly affects the development of follicles, can inhibit the growth of follicles, and prevent follicles from being consumed too quickly and prematurely , to preserve the reserve function of the ovary. [0003] In the prior art, there are few methods for measuring Müllerian inhibitor receptors. In view of this, it is necess...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/543G01N21/76
CPCG01N33/689G01N33/577G01N33/54326G01N21/76
Inventor 张旭东李冬梅孟令敏高威孙成艳
Owner DIRUI MEDICAL TECH CO LTD
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