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Purpose of tetrahedral frame nucleic acid in medicine for treating multiple sclerosis

A tetrahedron and drug technology, applied in the field of biomedicine, can solve problems such as unsatisfactory results, achieve abnormal activation and proliferation mitigation, enrich the number, and restore expression

Active Publication Date: 2021-08-20
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Remyelination has been proven effective in acute animal models of MS, such as the monoclonal antibody opicinumab (which targets LINGO1), antimuscarinic drugs such as clemastine and erythropoietin, but the results of these early phase clinical trials are not very clear. ideal

Method used

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  • Purpose of tetrahedral frame nucleic acid in medicine for treating multiple sclerosis
  • Purpose of tetrahedral frame nucleic acid in medicine for treating multiple sclerosis
  • Purpose of tetrahedral frame nucleic acid in medicine for treating multiple sclerosis

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Embodiment 1, the synthesis of DNA tetrahedron

[0037] Dissolve the four DNA single strands (S1, S2, S3, S4) in TM Buffer (10mM Tris-HCl, 50mM MgCl2, pH=8.0), the final concentration of the four DNA single strands is 1μM, mix well, and rapidly heat to 95 Keep the temperature at ℃ for 10 minutes, then quickly cool down to 4℃ and keep it for more than 20 minutes to obtain tetrahedral framework nucleic acid.

[0038] The sequences of the four single strands are as follows:

[0039]

[0040] Synthesized tetrahedral framework nucleic acid, PAGE gel electrophoresis ( figure 1 ) and capillary electrophoresis ( figure 2 ) It can be seen that the size of the tetrahedral framework nucleic acid is about 180KD, and it can be considered that the tetrahedral framework nucleic acid has been successfully synthesized. Tetrahedral particle size is about 10.15d.nm ( image 3 ), the potential is -2.83mV ( Figure 4 ). Using a transmission electron microscope, the tetrahedral str...

experiment example 1

[0042] Experimental example 1, the therapeutic effect of tetrahedral framework nucleic acid on multiple sclerosis (demyelinating disease) model

[0043] 1. Experimental method

[0044] Model animals: C57BL / 6J mice induced by cuprizone (CPZ) were used to prepare MS models of demyelination, and the effect and possible mechanism of tetrahedral framework nucleic acid promoting myelin regeneration and repair were explored. A total of 25 C57BL / 6 male mice aged 6-8 weeks were selected. Divided into normal group 8, control group 9, treatment group 8. The normal group was fed with common feed every day, and the control group and treatment group were fed with mixed feed containing 0.2% CPZ every day. After 6 weeks of feeding, the treatment group and the control group were respectively injected with tetrahedral framework nucleic acid (1000nM) and normal saline 0.1mL by tail vein, once every other day, a total of 4 times.

[0045] Relevant index detection: open-field experiments were p...

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Abstract

The invention provides a purpose of a tetrahedral frame nucleic acid in a medicine for treating demyelinating diseases, including multiple sclerosis. The tetrahedral frame nucleic acid is formed in a way that four single-stranded DNAs are subjected to complementary base pairing, and the sequences of the four single-stranded DNAs are independently selected from the sequences of SEQ ID NO.1-4 one by one. The tetrahedral frame nucleic acid can inhibit cell apoptosis of the central nervous system and recover expression of a myelin-associated protein so as to quicken re-myelination and increase an amount of myelinated axons. Therefore, the tetrahedral frame nucleic acid can effectively treat the demyelinating disease, especially the multiple sclerosis, and has an excellent clinical application prospect.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to the use of tetrahedral framework nucleic acid in medicine for treating demyelinating diseases, such as multiple sclerosis. Background technique [0002] Demyelinating disease is a kind of acquired disease with different etiology and different clinical manifestations, but has similar characteristics. The characteristic pathological change is demyelination of nerve fibers while nerve cells remain relatively intact. The role of myelin is to protect neurons and enable nerve impulses to be transmitted quickly on neurons, so the loss of myelin will affect the transmission of nerve impulses. [0003] As a disease with acute attack or subacute damage to the nerve center, if the treatment is delayed, multiple sclerosis will occur due to secondary ischemic degeneration of the damaged nerve. Multiple sclerosis (MS) is one of the most common demyelinating diseases, which has affected 2...

Claims

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Application Information

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IPC IPC(8): A61K31/713A61P25/28A61P25/00
CPCA61K31/713A61P25/28A61P25/00
Inventor 林云锋杨雨婷马文娟陈兴宇谢瑜
Owner SICHUAN UNIV
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