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Toad skin steroid total lactone extract, steroid lactone monomeric compound and application of toad skin steroid total lactone extract and steroid lactone monomeric compound in anti-influenza virus

An anti-influenza virus, total lactone technology, applied in the direction of steroids, antiviral agents, medical preparations containing active ingredients, etc., can solve problems such as no anti-influenza virus activity yet

Pending Publication Date: 2021-08-20
HEFEI HUAFANG PHARMA SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] So far, there has been no report that the total lactone extract of bufasterene extracted from toad skin and the monomeric compounds isolated therefrom have anti-influenza virus activity

Method used

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  • Toad skin steroid total lactone extract, steroid lactone monomeric compound and application of toad skin steroid total lactone extract and steroid lactone monomeric compound in anti-influenza virus
  • Toad skin steroid total lactone extract, steroid lactone monomeric compound and application of toad skin steroid total lactone extract and steroid lactone monomeric compound in anti-influenza virus
  • Toad skin steroid total lactone extract, steroid lactone monomeric compound and application of toad skin steroid total lactone extract and steroid lactone monomeric compound in anti-influenza virus

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Embodiment 1

[0053] The extraction of the total lactone of embodiment 1 bufacyl and the separation of monomer compound

[0054] Dry toad skin extract about 1200g, 800g silica gel mixing sample, silica gel column dry loading, column dry loading, cyclohexane-acetone gradient elution (10:1-8:1-5:1-2:1 ), collect the 5:1 eluted fraction, then mix the sample with 12g of silica gel, dry-load the silica gel column, dry-load the sample, and elute with cyclohexane-acetone gradient (10:1-8:1-5:1 -2:1), collected the eluted fraction of 5:1, recovered the solvent and dried to obtain 3.4g of the product, the total lactone content detected by HPLC method was 57.9% (repobufagenin: 0, cinobufagin: 33.3% , Bufalin: 24.6%); for the NMR data of cinobuffoni and bufafolin, see Figure 1-Figure 4 .

[0055] Separation and purification of monomeric compounds: Combine the remaining fractions, mix the samples with silica gel, dry-load on the silica gel column, and elute with the gradient of n-hexane-ethyl acetat...

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Abstract

The invention discloses a toad skin steroid total lactone extract, a steroid lactone monomeric compound and application of the toad skin steroid total lactone extract and the steroid lactone monomeric compound in anti-influenza virus. The toad skin steroid total lactone extract is prepared from a dry toad skin extract, and a plurality of steroid total lactone monomeric compounds are separated from an ethanol extract of bufo bufo gargarizans skin by means of repeated silica gel column chromatography, semi-preparative high performance liquid chromatography, recrystallization and the like. H7N9 subtype avian influenza strain in-vitro infection MDCK cells are adopted as a model, and ICR mice are adopted as an in-vivo model, so that in-vivo and in-vitro anti-influenza virus drug effects of the toad-toad skin steroid total lactone extract and the steroid lactone monomer compound are evaluated. The result shows that the toad skin steroid total lactone extract and the steroid lactone monomeric compound have obvious influenza virus inhibition activity.

Description

technical field [0001] The present invention relates to the extract of total sterene lactones, in particular to the extract of total sterene lactones and the isolated sterene lactone monomer compound. The present invention further relates to the extract of total sterene lactones and the isolated The invention relates to the use of enene lactone monomer compounds in the preparation of anti-influenza virus drugs, and belongs to the field of bubadenolactone total lactone extracts, monomer compounds and their anti-influenza virus applications. Background technique [0002] The toad skin is the dried skin of Bufo bufo gargarizans Cantor or Bufomelanostictus Schneider, which are Bufoidae animals. It is cool in nature, pungent in taste, slightly poisonous, and returns to the liver, spleen, and lung meridians. It has the effects of clearing away heat and detoxification, diuresis and swelling, relieving cough and asthma, diuresis and dissipating swelling. It is used for carbuncle, ...

Claims

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Application Information

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IPC IPC(8): C07J19/00C07J71/00A61K31/585A61P31/16A61K35/65
CPCC07J19/00C07J71/0021A61K31/585A61P31/16A61K35/65
Inventor 何勇杨士伟高永好彭扶云桂双英何宁吴宗好
Owner HEFEI HUAFANG PHARMA SCI & TECH
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