A kind of method and obtained product from aqueous phase extraction fat body in tomato seed
A technology for extracting oil and tomato seeds, which is applied in the direction of fat oil/fat production, fat oil/fat refining, fat production, etc. It can solve the problems of low purity of fat body and long extraction time, and achieve the goal of improving extraction rate and long shelf life Effect
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Embodiment 1
[0040] For the processing procedure, see figure 1 .
[0041] Take 20g of dried tomato seeds, soak in 0.1mol / L NaHCO with pH=9.50 3 In the solution, the soaking temperature is 4°C, the soaking time is 4 hours, and the soaking material-liquid ratio is 1:4 (w / v). The soaked seeds and soaking liquid are processed with a blender for 45 seconds, twice. Then filter with three layers of gauze to obtain filtrate and filter residue. The filtrate was centrifuged at 10,000 g at 4°C for 30 min, and the upper layer of paste was the fat body.
[0042] Dilute the oil body with deionized water at 1:10, and then dilute it 10 times, measure the particle size and ζ-potential of the oil body with the diluent, measure the particle size and ζ-potential of the oil body with a laser particle size analyzer, and set the equilibrium time for 90s , the measurement temperature was 25°C, each sample was shaken with a vortex mixer for 20 s before each sample was placed, and each sample was measured three...
Embodiment 2
[0044] Take 20g of dried tomato seeds, soak in 0.1mol / L NaHCO with pH=9.50 3In the solution, the soaking temperature is 4°C, the soaking time is 4 hours, and the soaking material-liquid ratio is 1:4 (w / v). Then use a twin-screw press to process three times, the first time to process the seeds and their soaking liquid, the second time to process the mixture of filtrate and residue, and the third time to process only the filtrate to obtain filtrate and filter residue. The filtrate was centrifuged at 10,000 g at 4°C for 30 min, and the upper layer of paste was the fat body.
[0045] Dilute the oil body with deionized water at 1:10, and then dilute it 10 times, measure the particle size and ζ-potential of the oil body with the diluent, measure the particle size and ζ-potential of the oil body with a laser particle size analyzer, and set the equilibrium time for 90s , the measurement temperature was 25°C, each sample was shaken with a vortex mixer for 20 s before each sample was p...
Embodiment 3
[0047] Take 20g of dried tomato seeds, soak in 0.1mol / L NaHCO with pH=9.50 3 In the solution, the soaking temperature is 4°C, the soaking time is 4 hours, and the soaking material-liquid ratio is 1:4 (w / v). Process the soaked tomato seeds and their soaking liquid with a blender for 45 seconds twice to obtain a once-treated slurry. Then the slurry is processed three times with a twin-screw press, the slurry is processed for the first time, the mixture of filtrate and residue is processed for the second time, and only the filtrate is processed for the third time to obtain the final extract. The extract was centrifuged at 10,000 g at 4°C for 30 min, and the upper paste was the fat body.
[0048] Dilute the oil body with deionized water at 1:10, and then dilute it 10 times, measure the particle size and ζ-potential of the oil body with the diluent, measure the particle size and ζ-potential of the oil body with a laser particle size analyzer, and set the equilibrium time for 90s ...
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