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Spirulina wall breaking method

A technology of spirulina and spirulina powder, applied in the biological field, can solve the problems of high energy consumption, long time consumption, and difficulty in industrial application, and achieve the effects of extending shelf life, improving stability, and high safety

Pending Publication Date: 2021-08-20
珠海康龙源进出口有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In view of the ubiquitous problems of long time consumption, high energy consumption and difficulty in industrial application of current spirulina wall breaking technology, the purpose of the present invention is to provide a new simpler and faster spirulina wall breaking method

Method used

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  • Spirulina wall breaking method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, a kind of spirulina wall breaking method

[0030] The method for breaking the wall of spirulina comprises the following steps:

[0031] S1 using 10mM citric acid solution to adjust the pH of 10mM sodium citrate solution to 6.5 to obtain 10mM citric acid-sodium citrate buffer;

[0032] S2 Weigh 10.0g of spirulina powder and 400g of the buffer solution obtained in step S1 respectively, and slowly add the spirulina powder into the buffer solution under the condition of 30°C and 300r / min magnetic stirring state to avoid agglomeration of the spirulina powder. Stir for 3 hours to obtain the extract of spirulina after wall breaking.

Embodiment 2

[0033] Embodiment 2, a kind of spirulina wall breaking method

[0034] The method for breaking the wall of spirulina comprises the following steps:

[0035] S1 prepares a citric acid-sodium citrate buffer solution with a concentration of 50 mM and a pH of 6.8;

[0036] S2 Weigh 10.0g of spirulina powder and 200g of the buffer solution obtained in step S1 respectively, and slowly add the spirulina powder into the buffer solution under the condition of 10°C and 600r / min magnetic stirring state to avoid agglomeration of the spirulina powder. Stir for 4 hours to obtain the extract of spirulina after wall breaking.

Embodiment 3

[0037] Embodiment 3, a kind of spirulina wall breaking method

[0038] The method for breaking the wall of spirulina comprises the following steps:

[0039] S1 prepares a citric acid-potassium citrate buffer solution with a concentration of 20 mM and a pH of 6.6;

[0040] S2 Weigh 10.0g of spirulina powder and 500g of the buffer solution obtained in step S1 respectively, and slowly add the spirulina powder into the buffer solution under the condition of 20°C and 900r / min magnetic stirring state to avoid agglomeration of the spirulina powder. Stir for 2 hours to obtain the extract of spirulina after wall breaking.

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a method for rapid wall breaking of spirulina to extract natural products. The spirulina wall breaking method provided by the invention comprises the following steps: S1, preparing a buffer solution with the concentration of 5-200 mM and the pH value of 6.0-7.5 by using polycarboxylic acids and salts thereof in a specific ratio; S2, separately weighing spirulina powder and the buffer solution obtained in the step S1, slowly adding the spirulina powder into the buffer solution at a certain stirring speed under the condition of 10-30 DEG C to avoid caking of the spirulina powder, and continuously stirring the mixture to obtain a wall-broken spirulina extracting solution. The spirulina wall breaking method provided by the invention is simpler and quicker, and is easy for industrialization implementation.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for rapidly breaking the wall of spirulina to extract natural products. Background technique [0002] Spirulina is rich in phycobiliproteins, polysaccharides, γ-linolenic acid, vitamins, carotene and other bioactive substances, and is an ideal food for human beings. Phycobiliproteins include phycocyanin, allophycocyanin, phycoerythrin, and phycoerythrin; among them, phycocyanin is an effective antioxidant that has the effect of scavenging free radicals and anti-inflammation, and it is also used as a A rare natural blue colorant, it has good applications in chewing gum, ice cream, beverages, candies and cosmetics. Both the US FDA and the European Union have approved phycocyanin as a natural food coloring, and it can be used in unlimited quantities; the Chinese national standard GB2760 food additive catalog also allows phycocyanin to be used as a natural coloring ...

Claims

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Application Information

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IPC IPC(8): C12N1/06
CPCC12N1/066
Inventor 陈文田李海霞薛升长同敏忠
Owner 珠海康龙源进出口有限公司