Rapid determination method of fatty acid and squalene in plant oil
A rapid determination of vegetable oil technology, applied in the field of analytical chemistry, can solve the problems of toxic reagents, waste of resources, high cost, etc., to achieve the effect of rapid method, resource saving, and solvent cost reduction
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Embodiment 1
[0017] A rapid determination method of fatty acid and squalene in olive oil, which is characterized by going through the following steps:
[0018] A. According to the amount of 35mL / g olive oil, add 2ml of n-hexane to 40mg olive oil to completely dissolve the olive oil, and then add the concentration of 2mol / L potassium hydroxide and 2mol / L according to the amount of 5.5mL / g olive oil. 0.3 mL of methanol mixed solution, shake for 3 minutes, centrifuge for 5 minutes, and get the upper sample solution;
[0019] B. Send the sample liquid obtained in step A to a gas chromatograph for GC and MS analysis, and calculate the percentage of each fatty acid component and functional component squalene in olive oil according to the conventional area normalization method, respectively Is: total fatty acid content 94.10% (respectively: hexadecenoic acid 1.05%, palmitic acid 14.14%, linoleic acid 16.01%, oleic acid 60.44%, stearic acid 1.50%, eicosenoic acid 0.29%, arachidic acid Acid 0.30%, unkn...
Embodiment 2
[0024] A rapid determination method of fatty acid and squalene in olive oil, which is characterized by going through the following steps:
[0025] A. According to the amount of 40mL / g olive oil, add 2mL of n-hexane into 50mg olive oil to dissolve the olive oil completely, and then add 2mol / L potassium hydroxide and methanol according to the amount of 6mL / g olive oil. The mixed solution of 0.3mL, shake for 4min, centrifuge for 6min, get the upper sample solution;
[0026] B. Send the sample liquid obtained in step A to a gas chromatograph for GC and MS analysis, and calculate the percentage of each fatty acid component and functional component squalene in olive oil according to the conventional area normalization method, respectively Is: fatty acid content 91.70% (respectively: hexadecenoic acid 1.36%, palmitic acid 15.18%, linoleic acid 10.14%, oleic acid 62.33%, stearic acid 1.58%, eicosenoic acid 0.31%, arachidic acid 0.33%, unknown 0.17%, behenic acid 0.30%); squalene content 8...
Embodiment 3
[0031] A rapid determination method of fatty acid and squalene in olive oil, which is characterized by going through the following steps:
[0032] A. According to the amount of 50mL / g olive oil, add 2mL of n-hexane into 60mg olive oil to completely dissolve the olive oil, and then add 2mol / L potassium hydroxide and 2mol / L according to the amount of 7.5mL / g olive oil. 0.3 mL of methanol mixed solution, shake for 5 minutes, centrifuge for 8 minutes to obtain the upper layer sample solution;
[0033] B. Send the sample liquid obtained in step A to a gas chromatograph for GC and MS analysis, and calculate the percentage of each fatty acid component and functional component squalene in olive oil according to the conventional area normalization method, respectively Is: fatty acid content 95.97% (respectively: hexadecenoic acid 1.07%, palmitic acid 14.10%, linoleic acid 8.01%, oleic acid 69.99%, stearic acid 1.60%, eicosenoic acid 0.24%, arachidic acid 0.32%, unknown 0.24%, behenic acid ...
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