Rapid determination method of fatty acid and squalene in plant oil

A rapid determination of vegetable oil technology, applied in the field of analytical chemistry, can solve the problems of toxic reagents, waste of resources, high cost, etc., to achieve the effect of rapid method, resource saving, and solvent cost reduction

Active Publication Date: 2013-06-12
YUNNAN ACAD OF FORESTRY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the cumbersome and complicated operation in the method of vegetable oil fatty acid methyl esterification in the prior art, time-consuming and labor-intensive, and the use of harmful and toxic reagents, high cost and serious waste of resources, the present invention provides a convenient, fast, The cost is low, the sample only needs one pre-treatment, and the same chromatographic column can be used for the separation and determination of the main fatty acid and functional component squalene in vegetable oil

Method used

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  • Rapid determination method of fatty acid and squalene in plant oil

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Experimental program
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Effect test

Embodiment 1

[0017] A rapid determination method of fatty acid and squalene in olive oil, which is characterized by going through the following steps:

[0018] A. According to the amount of 35mL / g olive oil, add 2ml of n-hexane to 40mg olive oil to completely dissolve the olive oil, and then add the concentration of 2mol / L potassium hydroxide and 2mol / L according to the amount of 5.5mL / g olive oil. 0.3 mL of methanol mixed solution, shake for 3 minutes, centrifuge for 5 minutes, and get the upper sample solution;

[0019] B. Send the sample liquid obtained in step A to a gas chromatograph for GC and MS analysis, and calculate the percentage of each fatty acid component and functional component squalene in olive oil according to the conventional area normalization method, respectively Is: total fatty acid content 94.10% (respectively: hexadecenoic acid 1.05%, palmitic acid 14.14%, linoleic acid 16.01%, oleic acid 60.44%, stearic acid 1.50%, eicosenoic acid 0.29%, arachidic acid Acid 0.30%, unkn...

Embodiment 2

[0024] A rapid determination method of fatty acid and squalene in olive oil, which is characterized by going through the following steps:

[0025] A. According to the amount of 40mL / g olive oil, add 2mL of n-hexane into 50mg olive oil to dissolve the olive oil completely, and then add 2mol / L potassium hydroxide and methanol according to the amount of 6mL / g olive oil. The mixed solution of 0.3mL, shake for 4min, centrifuge for 6min, get the upper sample solution;

[0026] B. Send the sample liquid obtained in step A to a gas chromatograph for GC and MS analysis, and calculate the percentage of each fatty acid component and functional component squalene in olive oil according to the conventional area normalization method, respectively Is: fatty acid content 91.70% (respectively: hexadecenoic acid 1.36%, palmitic acid 15.18%, linoleic acid 10.14%, oleic acid 62.33%, stearic acid 1.58%, eicosenoic acid 0.31%, arachidic acid 0.33%, unknown 0.17%, behenic acid 0.30%); squalene content 8...

Embodiment 3

[0031] A rapid determination method of fatty acid and squalene in olive oil, which is characterized by going through the following steps:

[0032] A. According to the amount of 50mL / g olive oil, add 2mL of n-hexane into 60mg olive oil to completely dissolve the olive oil, and then add 2mol / L potassium hydroxide and 2mol / L according to the amount of 7.5mL / g olive oil. 0.3 mL of methanol mixed solution, shake for 5 minutes, centrifuge for 8 minutes to obtain the upper layer sample solution;

[0033] B. Send the sample liquid obtained in step A to a gas chromatograph for GC and MS analysis, and calculate the percentage of each fatty acid component and functional component squalene in olive oil according to the conventional area normalization method, respectively Is: fatty acid content 95.97% (respectively: hexadecenoic acid 1.07%, palmitic acid 14.10%, linoleic acid 8.01%, oleic acid 69.99%, stearic acid 1.60%, eicosenoic acid 0.24%, arachidic acid 0.32%, unknown 0.24%, behenic acid ...

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Abstract

The invention provides a rapid determination method of fatty acid and squalene in plant oil. The method comprises the following steps of: adding n-hexane in the plant oil according to 35-50mL / g of plant oil to fully dissolve the plant oil; adding a mixed solution of potassium hydroxide and methanol according to 5.5-7.5mL / g of plant oil, oscillating for 3-5 minutes, and carrying out centrifugal separation for 5-8 minutes to obtain an upper sampling solution, wherein the concentration of the mixed solution is 2mol / L; and carrying out GC (gas chromatography) and MS (mass spectrum) analysis in a gas supply phase chromatographic instrument, and calculating according to a conventional area normalization method to obtain the components of the fatty acid and the percentage composition of the functional ingredient squalene. The extraction of sample fatty acid and the methyl esterification of the fatty acid are accomplished synchronously, and at the same time, squalene is extracted in a solvent well, so that the method is rapid, simple and less in solvent amount, and has no harm to human health, therefore, the lower detection limit is realized. The method is applicable to the detection of fatty acid and squalene contents in any plant oil.

Description

[0001] Technical field [0002] The invention relates to a method for determining plant components, in particular to a method for quickly determining fatty acids and squalene in olive oil, belonging to the technical field of analytical chemistry. technical background [0003] In plant extraction and plant component analysis, the determination of main fatty acids and functional components in vegetable oil is often involved. The existing technical standards all use cumbersome and complicated operations and require a variety of toxic reagents (such as concentrated sulfuric acid, petroleum ether, benzene, ether, etc.) to cooperate with methyl esterification methods for the pretreatment of olive oil. The effect is not satisfactory. When the processed olive oil is sent to the gas chromatograph for analysis, the chromatographic peak will not appear, or the separation effect of the two main components of fatty acid and squalene is not ideal, and the main fatty acid in olive oil will be se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 耿树香张艳丽李勇杰马婷肖良俊谷丽萍贺娜王洋廖永坚陈海云徐玉梅宁德鲁
Owner YUNNAN ACAD OF FORESTRY
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