Mesenchymal stem cell exosome agent based on DNA tetrahedron, preparation method and application of mesenchymal stem cell exosome agent

A stromal stem cell and tetrahedral technology, which is applied in the field of mesenchymal stem cell exosome preparations and their preparation, and can solve problems such as toxic and side effects

Active Publication Date: 2021-08-24
HEBEI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, a large amount of drugs still reach normal tissues, causing serious toxic side effects

Method used

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  • Mesenchymal stem cell exosome agent based on DNA tetrahedron, preparation method and application of mesenchymal stem cell exosome agent
  • Mesenchymal stem cell exosome agent based on DNA tetrahedron, preparation method and application of mesenchymal stem cell exosome agent
  • Mesenchymal stem cell exosome agent based on DNA tetrahedron, preparation method and application of mesenchymal stem cell exosome agent

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Experimental program
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Embodiment 1

[0046] The preparation method of Exo-TDN and PCM-Exo-TDN nano material, such as figure 1 shown, including the following steps:

[0047] (1) Isolation of rat bone marrow mesenchymal stem cells

[0048] 3-week-old healthy female SD rats were selected, killed by cervical dislocation, and then immersed in prepared 75% ethanol for 10 min. Take the rat out of 75% ethanol, put it in a super-clean table, cut the skin of the leg of the rat with sterile forceps and scissors, take out the femur and tibia and put them in PBS containing 1% double antibody. Scrape off the muscles, tendons and connective tissues on the surface of the femur and tibia with a scalpel blade in PBS, cut off the epiphyses at both ends of the femur and tibia to fully leak out the bone cavity, and place them in DMEM containing 10% FBS and 1% double antibody for culture In the base, use a 1mL syringe to absorb the culture medium and then insert it into the bone marrow cavity, and blow out the bone marrow until the ...

Embodiment 2

[0069] (1) The function of Exo-TDN in protecting cells in vitro

[0070]In order to verify the killing effect of doxorubicin on cancer cells and the toxicity to normal cells, we verified the killing effect of different concentrations of doxorubicin on cancer cells by MTT method, as shown in Figure 8 As shown, doxorubicin has a strong killing effect on MCF-7. When 0.4μM doxorubicin, the survival rate of MCF-7 is only about 50%, which has a good effect on inhibiting the proliferation of cancer cells. However, doxorubicin 3A is also toxic to normal liver cells. Under the action of 1 μM doxorubicin, the proliferation of liver cells is greatly inhibited, indicating that doxorubicin has certain toxic side effects on normal cells while killing tumor cells . Next, we verified whether different concentrations of tetrahedral DNA and different concentrations of exosomes have toxic side effects on cells. As shown in the figure, different concentrations of tetrahedral DNA have no toxic e...

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Abstract

The invention provides a mesenchymal stem cell exosome agent based on a DNA tetrahedron, a preparation method and application of the mesenchymal stem cell exosome agent. The exosome preparation is an exosome agent Exo-TDN which is obtained by loading a DNA tetrahedral nano material on the surface of a mesenchymal stem cell exosome; or the exosome agent is an exosome preparation PCM-Exo-TDN which is obtained by loading a DNA tetrahedral nano material on a mesenchymal stem cell exosome and modifying a myocardial specific targeting peptide PCM on the surface of the exosome through a cross-linking agent phospholipid polyethylene glycol maleimide. Exo-TDN is mainly enriched in a liver area, the chemotherapeutic drug DOX can be captured by the Exo-TDN when reaching the liver, the DOX is inhibited from entering a cell nucleus, and the function of protecting cells is achieved; a myocardial specific targeting peptide PCM is further modified, and PCM-Exo-TDN is synthesized and used for relieving myocardial damage caused by DOX.

Description

technical field [0001] The invention relates to the technical field of detoxification and repairing agents for tumor chemotherapy, in particular to a DNA tetrahedron-based mesenchymal stem cell exosome preparation and its preparation method and application. Background technique [0002] Malignant tumors (commonly known as cancer) are the leading public health problems worldwide, posing a continuous challenge to human health. Despite the continuous development of emerging methods including immunotherapy, photothermal therapy, and photodynamic therapy, the most important means of clinical cancer treatment is still chemotherapy based on chemical drugs. However, due to the non-specific distribution of chemotherapeutic drugs, which usually cause severe toxic side effects, it has attracted extensive attention. The liver plays a central role in drug metabolism and clearance. Drug-induced liver injury (DILI) is the main cause of acute liver failure, and the occurrence of liver inju...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/46A61K31/711A61K47/64A61K47/69A61K35/28A61P35/00A61P39/02A61P1/16A61P9/00C12N5/0775
CPCA61K47/46A61K31/711A61K47/64A61K47/6931A61K35/28A61P35/00A61P39/02A61P1/16A61P9/00C12N5/0662A61K2300/00
Inventor 李振华张金超李航刘会芳
Owner HEBEI UNIVERSITY
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