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Preparation method of microbial agent for soil acidification area

A microbial agent and soil acidification technology, applied in the direction of microorganism-based methods, methods of supporting/immobilizing microorganisms, biochemical equipment and methods, etc., can solve the problems of reducing the effect and function of microbial agents, microbial death, etc., and achieve survival The effect of improving the efficiency, improving the preparation efficiency, and strong practicability

Active Publication Date: 2021-09-03
沈阳华清源农业发展有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] When the microbial bacterial agents in the prior art are used in areas with serious soil acidification, because the soil is acidic, the microorganisms in the microbial agents are likely to die in large quantities because they cannot adapt to the harsh soil environment, which will greatly reduce the microbial growth rate. The effect and action of the agent

Method used

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  • Preparation method of microbial agent for soil acidification area
  • Preparation method of microbial agent for soil acidification area
  • Preparation method of microbial agent for soil acidification area

Examples

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Embodiment 1

[0044] see figure 1 with Image 6 , a preparation method of microbial bacterial agent for soil acidification area, comprising the following steps:

[0045] S1. Take an appropriate amount of in-situ soil in the target area as a soil sample, remove impurities from the soil sample, remove impurities and garbage in the soil sample, and obtain a soil sample without impurities;

[0046] S2. Carrying out high-temperature sterilization on the de-impured soil sample to obtain a sterilized de-impured soil sample, the sterilization temperature is 90-130° C., and the sterilization time is 20-40 min;

[0047] S3. Thoroughly mix and stir the sterilized and decontaminated soil sample and water, the mass ratio of the sterilized and decontaminated soil sample to water is 1:1-2, after natural precipitation, take the supernatant as the screening liquid;

[0048] S4. Pour the screening solution into the screening box 002, then put beneficial microbial strains and liquid culture medium into the ...

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Abstract

The invention discloses a preparation method of a microbial agent for a soil acidification region, and belongs to the technical field of microbial agents. The method creatively adds a procedure of performing screening culture on strains by using soil in a target region, before the microbial agent is prepared, sampling is performed in the target region, a soil sample is subjected to impurity removal, sterilization, water adding and mixing to prepare a screening solution, the strains are screened through the screening solution, and high-quality strains capable of surviving in the screening solution, namely strains capable of adapting to the soil environment of the target region are screened out, so that when the prepared microbial agent is used in the target region, the survival rate of microorganisms in the microbial agent can be greatly improved, the effect of the microbial agent is further effectively improved, practicability is high, microbial collection balls used for collecting high-quality strains are arranged, and the microbial collection balls can rapidly and effectively collect the screened high-quality strains based on chemotaxis of the microorganisms, so that the preparation efficiency can be effectively improved.

Description

technical field [0001] The invention relates to the technical field of microbial inoculum, in particular to a method for preparing microbial inoculum for soil acidification areas. Background technique [0002] Microbial agent refers to the live bacterial preparation made by processing the fermentation broth of the target microorganisms (effective bacteria) by using porous substances as adsorbents (such as peat and vermiculite) after industrial production and expansion. This kind of bacterial agent is used for seed dressing or root dipping, and has the functions of directly or indirectly improving soil, restoring soil fertility, preventing soil-borne diseases, maintaining the balance of rhizosphere microbial flora, and degrading toxic substances. Proper use of agricultural microbial bacterial agents can improve The output of agricultural products, improving the quality of agricultural products, reducing the amount of chemical fertilizers, reducing costs, improving soil, and p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C09K17/14C09K17/32C12M1/12C12M1/00C12R1/41C12R1/07
CPCC12N1/20C12N1/16C09K17/14C09K17/32C12M23/38C12M23/48C12M23/50C12M33/12C12M33/14C12M37/04Y02W30/40
Inventor 李雪琛
Owner 沈阳华清源农业发展有限公司
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