Bifunctional exosome, preparation method thereof and application in cerebral apoplexy repair
An exosome and dual-function technology, applied in biochemical equipment and methods, cell dissociation methods, pharmaceutical formulations, etc., can solve the problems of cell toxicity, interference with protein activity, etc., to increase neurogenesis, promote neural differentiation, Improve the effect of accumulation
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[0039] In order to make the objects, technical solutions and advantages of the present invention, the present invention will be specifically described below, it is necessary to explain and explain the present invention, and is not intended to limit the invention. . Some non-nature improvements and adjustments made by those skilled in the art are still within the scope of the invention.
[0040] Implementation
[0041] First, BV2 small colloidal cell culture, polarization and identification
[0042] Cells were cultured in a DMEM medium containing 10% FBS (fetal bovine serum) and 1% penicillin formation in a 37 ° C incubator. The BV2 M2 polarization method is as follows: IBA-1, CD206 and Arg antibodies were detected by Treatment of BV2 cells with 20 ng / ml of IL-4 (interleukin). WesternBlotting (immunoprotein blot) was used to detect IL-4 treated BV2 cells and unsuccessined BV2 cells CD206 and ARG expression.
[0043] Second, the exosomental separation
[0044] First, the FBS serum...
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