An aptamer molecular probe-modified porous microneedle patch and its preparation method and application

A molecular probe and microneedle sticking technology, which is applied in the field of biomedical materials, can solve the problems of complex microprocessing technology and high manufacturing cost, and achieve the effect of easy reaction technical conditions, strong repeatability, and improved detection efficiency

Active Publication Date: 2022-05-20
NANJING DRUM TOWER HOSPITAL
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, common microneedle arrays usually require complicated microfabrication techniques in the manufacturing process, the manufacturing cost is high, and post-processing such as bodily fluid recovery is often required

Method used

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  • An aptamer molecular probe-modified porous microneedle patch and its preparation method and application
  • An aptamer molecular probe-modified porous microneedle patch and its preparation method and application
  • An aptamer molecular probe-modified porous microneedle patch and its preparation method and application

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Embodiment Construction

[0033] The present invention will be further described below in conjunction with specific examples. The experimental methods used in the following examples, unless otherwise specified, are conventional methods, and the reagents, methods and equipment used, unless otherwise specified, are conventional reagents, methods and equipment in the art.

[0034] The present invention provides a porous microneedle patch modified by an aptamer molecular probe for endotoxin detection using dopamine as a biological adhesive, which is prepared according to the following method:

[0035] Step 1. Preparation of PDMS needle-like hole microneedle array template: PDMS and curing agent are prepared into PDMS to be cured at a mass ratio of 10:1, and then the PDMS to be cured is evacuated for 20 minutes for pre-degassing treatment; The degassed PDMS to be cured is poured on the surface of the ETPTA microneedle array positive template; the system is evacuated again for 15 minutes, and then heated and...

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Abstract

The invention discloses a porous microneedle patch modified by an aptamer molecular probe and its preparation method and application. The preparation method comprises the following steps: step 1, preparing a PDMS needle-shaped hole microneedle array template; step 2, making The surface of the pore agent is coated with a layer of bio-adhesive to obtain a pore-making material; step 3, the pore-forming liquid and the microneedle raw material solution are poured step by step into the PDMS needle-like hole microneedle array template, and the pore-forming material is prepared. After the pore liquid and the microneedle raw material solution are solidified, peel off to obtain a microneedle patch containing a pore-forming material; step 4, prepare a porous microneedle patch: combine the microneedle patch containing a pore-forming material with a pore-forming etchant Carry out the reaction to form holes and make a porous microneedle patch; Step 5, prepare the porous microneedle patch modified by the aptamer molecular probe: react the porous microneedle patch and the aptamer molecular probe in the buffer , to obtain the porous microneedle patch modified by the aptamer molecular probe. The invention has the advantages of simplicity, convenience, high efficiency and the like.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and relates to a microneedle patch, in particular to a porous microneedle patch modified by an aptamer molecular probe, a preparation method and an application thereof. Background technique [0002] With the advancement of modern medicine and the improvement of people's living standards, people's demand for clinical testing is increasing day by day. Body fluid testing is a relatively common type of clinical testing, of which blood testing accounts for the largest proportion. Sampling for blood testing is usually done using a syringe or vacuum blood collection tube. But this sampling method often brings pain and psychological stress to patients, and these effects may cause some physiological indicators to fluctuate, thereby reducing the accuracy of test results. Microneedle arrays are a very innovative microstructured material that has been developed for sampling body fluids in rece...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B29C70/58B29C33/38B29C33/40G01N21/64A61B5/00A61B5/145
CPCB29C70/58B29C33/3842B29C33/40G01N21/6428A61B5/685A61B5/14503A61B5/14514A61B5/14546
Inventor 赵远锦易可馨池俊杰王月桐商珞然
Owner NANJING DRUM TOWER HOSPITAL
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