Emulsion with stable liposome and preparation method thereof
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A liposome and stable technology, applied in the field of liposome-stabilized emulsion and its preparation, can solve the problems of poor thermodynamic stability and low loading rate, and achieve the effects of improving stability, promoting digestion and absorption, and preventing macular degeneration
Active Publication Date: 2021-09-10
QINGDAO AGRI UNIV
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The liposome-stabilized emulsion is to construct a liquid interface as an oil-in-water (O / W) emulsion of an emulsifier. At present, in the prior art, the liposome-stabilized emulsion has a lower load rate and poor thermodynamic stability. problems, which limits its application in food, especially health products, especially health drinks
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Embodiment 1
[0032] Step 1, preparation of liposome film: dissolve 10mg of vitamin E, 200mg of phospholipids, 50mg of cholesterol and 100mg of Tween 80 in 20mL of ethanol, and carry out rotary evaporation at 40°C and 0.085MPa until the ethanol is completely evaporated to obtain a transparent Liposome film;
[0033] Step 2, preparation of dispersion liquid: dissolve 20mg vitamin B2 in 40mL phosphate buffer (0.02mol / L, pH=7.4), add the liposome film prepared in step 1, at 40°C, 50% power Break for 15 minutes (total power 450W, every break for 3 seconds, stop for 1 second) until it is clear and transparent, and a dispersion containing both vitamin E and vitamin B2 is obtained;
[0034] Step 3, preparation of coarse emulsion: Dissolve 200mg of β-carotene and 2g of phospholipid in 10mL of corn oil, mix 40mL of the dispersion prepared in step 2 with 150mL of deionized water, and use a high-speed disperser to disperse at 10000rpm for 5min to obtain a coarse emulsion ;
[0035] Step 4, prepare l...
Embodiment 2
[0045] The difference from Example 1 is that the amount of dispersion liquid added in step 3 is 20 mL.
[0046] Result: Laser confocal microscopic observation was carried out on the prepared functional beverage, and the result was similar to that of Example 1, and the accompanying drawings will not be repeated here.
Embodiment 3
[0048] The difference from Example 1 is that the amount of dispersion liquid added in step 3 is 25 mL.
[0049] Result: Laser confocal microscopic observation was carried out on the prepared functional beverage, and the result was similar to that of Example 1, and the accompanying drawings will not be repeated here.
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Abstract
The invention discloses an emulsion with stable liposome and a preparation method thereof, and relates to the field of food processing. The preparation method of the emulsion with stable liposome comprises the following steps: preparing a liposome film, preparing a dispersion liquid, preparing a crude emulsion and preparing the emulsion with stable liposome. By reasonably proportioning the components of the liposome and optimizing the preparation steps and parameters, the loading rate of vitamin E, vitamin B2 and beta-carotene is improved, and meanwhile, the thermodynamic stability of the emulsion with stable liposome is also improved, so that the emulsion can be kept stable during ultrahigh-temperature instantaneous sterilization, the vitamin B2, vitamin E and beta-carotene are delivered in target, digestion and absorption are promoted, the effect of preventing maculopathy is achieved, and meanwhile the beneficial health-care function is achieved.
Description
technical field [0001] The invention relates to the field of food processing, in particular to a liposome-stabilized emulsion and a preparation method thereof. Background technique [0002] The macular area is an important area of the retina, located at the posterior pole of the eye, and is mainly related to visual functions such as fine vision and color vision. Once lesions appear in the macular area, vision loss, black shadows in front of the eyes or visual distortion often occur. Macular degeneration is a common disease affecting the macula in older adults, the exact cause of which is unknown. For people over the age of 65, macular degeneration is the number one cause of permanent blindness. Studies have shown that vitamin E can inhibit the lipid peroxide reaction in the lens of the eye, dilate peripheral blood vessels, improve blood circulation, prevent the occurrence and development of myopia, and play a certain role in retinal health and reduce the risk of macular ...
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