Method for preparing food-borne ACE inhibitory peptide through frequency sweep ultrasonic coupling enzymolysis

A swept-frequency ultrasound and peptide inhibition technology, which is applied in the preparation method of peptides, the function of food ingredients, chemical instruments and methods, etc., can solve the problems of low inhibition rate and so on.

Pending Publication Date: 2021-09-14
QINGDAO AGRI UNIV
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example Ren et al. (Ren X, Ma H, Mao S, et al. Effects of sweeping frequency ultrasound treatment on enzymematic preparations of ACE-inhibitory peptides from zein [J]. European Food Research & Technology, 2014, 238 (3): 435-442 .) The zein hydrolyzate prepared by treating zein with 40±2kHz swept-frequency ultrasound (SFU) has 42.9% ACE inhibitory activity, which is 116.7% higher than the control; Mao Shuyun et al. (Mao Shuyun, Ma Haile, Ren Xiaofeng, etc. .Effect of frequency-sweeping ultrasonic treatment on the enzymatic properties of zein[J].Food Industry Science and Technology, 2013,34(014):142-147,155.) Using dual

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing food-borne ACE inhibitory peptide through frequency sweep ultrasonic coupling enzymolysis
  • Method for preparing food-borne ACE inhibitory peptide through frequency sweep ultrasonic coupling enzymolysis
  • Method for preparing food-borne ACE inhibitory peptide through frequency sweep ultrasonic coupling enzymolysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A method for preparing food-derived ACE-inhibiting peptides through frequency-sweeping ultrasound coupled with enzymatic hydrolysis, the steps are as follows:

[0042](1) Preparation of zein suspension: Dissolve 4 g of zein in 100 mL of distilled water, stir well to disperse it evenly, and obtain a zein powder suspension.

[0043] (2) Frequency-sweeping ultrasonic treatment: set the ultrasonic temperature to 25°C, place the zein suspension in a frequency-sweeping ultrasonic cleaning machine, and process the zein suspension at a dual-frequency ultrasonic frequency of 25 / 33kHz, The ultrasonic time is 30min, the two frequencies are cycled repeatedly, and the frequency sweep cycle is 4min.

[0044] (3) Enzymatic hydrolysis: Add alkaline protease to the ultrasonically treated zein suspension, the addition amount is 2800U / g zein, the activity of alkaline protease is 200000U / g, under the condition of 50℃ water bath The reaction was stirred at a speed of 700r / min, the sample s...

Embodiment 2

[0048] A method for preparing food-derived ACE-inhibiting peptides through frequency-sweeping ultrasound coupled with enzymatic hydrolysis, the steps are as follows:

[0049] (1) Preparation of zein suspension: Dissolve 4 g of zein in 100 mL of distilled water, stir well to disperse it evenly, and obtain a zein powder suspension.

[0050] (2) Frequency-sweeping ultrasonic treatment: set the ultrasonic temperature to 25°C, place the zein suspension in a frequency-sweeping ultrasonic cleaning machine, and process the zein suspension at a dual-frequency ultrasonic frequency of 25 / 40kHz, The ultrasonic time is 30min, the two frequencies are cycled repeatedly, and the frequency sweep cycle is 4min.

[0051] (3) Enzymolysis: Add alkaline protease to the sonicated zein suspension, the addition amount is 3000U / g zein, the activity of alkaline protease is 200000U / g, under the condition of 48 ℃ water bath, The reaction was stirred at a speed of 680r / min, the sample solution was titrate...

Embodiment 3

[0055] A method for preparing food-derived ACE-inhibiting peptides through frequency-sweeping ultrasound coupled with enzymatic hydrolysis, the steps are as follows:

[0056] (1) Preparation of zein suspension: Dissolve 4 g of zein in 100 mL of distilled water, stir well to disperse it evenly, and obtain a zein powder suspension.

[0057] (2) Frequency-sweeping ultrasonic treatment: set the ultrasonic temperature to 25°C, place the zein suspension in a frequency-sweeping ultrasonic cleaning machine, and process the zein suspension at a dual-frequency ultrasonic frequency of 33 / 40kHz, The ultrasonic time is 30min, the two frequencies are cycled repeatedly, and the frequency sweep cycle is 4min.

[0058] (3) Enzymolysis: Add alkaline protease to the sonicated zein suspension, the addition amount is 3200U / g zein, the activity of alkaline protease is 200000U / g, under the condition of 46 ℃ water bath, The reaction was stirred at a speed of 660r / min, the sample solution was titrate...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for preparing food-borne ACE inhibitory peptide through frequency sweep ultrasonic coupling enzymolysis, and belongs to the technical field of preparation of food-borne ACE inhibitory peptide. The method for preparing food-borne ACE inhibitory peptide through frequency sweep ultrasonic coupling enzymolysis comprises the steps of carrying out frequency sweep ultrasonic treatment on zein, and then carrying out protease enzymolysis on the zein. The finally prepared food-borne ACE inhibitory peptide has high ACE inhibitory activity, and the highest ACE inhibitory activity can reach 81.77%; compared with traditional enzymolysis, the method has the advantage that the activity of the ACE inhibitory peptide is improved by 42.35%; and compared with a traditional ultrasonic-assisted enzymolysis method, the method has the advantage that the activity of the ACE inhibitory peptide is improved by 26.20%. Therefore, the food-borne ACE inhibitory peptide prepared through the method has wide application prospects in preparation of drugs, health care products, food or preparations with the blood pressure lowering effect.

Description

technical field [0001] The invention belongs to the technical field of preparation of food-derived ACE inhibitory peptides, and in particular relates to a method for preparing food-derived ACE inhibitory peptides through frequency-sweeping ultrasonic coupled enzymatic hydrolysis. Background technique [0002] Angiotensin-converting enzyme inhibitory peptides (ACE inhibitory peptides) are key peptides in blood pressure regulation. Most of the peptides used in clinical practice are chemically synthesized ACE inhibitory peptides. Long-term use has different effects on health. Obtaining ACE inhibitory peptides from food proteins to reduce the side effects of chemically synthesized ACE inhibitory peptides has attracted more and more attention from researchers. Compared with the existing chemically synthesized ACE-inhibiting peptide antihypertensive drugs, food-derived ACE-inhibiting peptide has significant advantages such as mild antihypertensive effect, safety, no side effects, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/30A23L33/18A61K38/01A61P9/12
CPCC12P21/06C07K1/36C07K1/34C07K1/30A23L33/18A61K38/011A61P9/12A23V2002/00A23V2200/326A23V2250/55
Inventor 朱英莲张爽郑文滨杨庆利
Owner QINGDAO AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap