Soybean and soybean oil producing place tracing method based on MALDI-TOF/TOF characterization triglyceride
A triglyceride and origin traceability technology, which is applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of difficulty in detection of restricted polypeptides, difficulty in traceability identification, difficulty in polypeptide detection, etc., to achieve perfect accuracy, simple and convenient operation , the effect of improving the accuracy
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Embodiment 1
[0051] Soybean samples from different origins with definite regions are physically pressed through an oil press to obtain standard samples, using 2,5-dihydroxybenzoic acid as the matrix, dipping the standard samples with cotton swabs and printing them on the target plate containing the matrix, every 6 Standard samples were calibrated once using PEG, and each standard sample was measured in parallel three times. The target plate is placed in the matrix-assisted laser desorption ionization time-of-flight mass spectrometer. The working conditions of the matrix-assisted laser desorption ionization time-of-flight mass spectrometer are: the mass spectrometer adopts positive ion mode to collect data, the ion source voltage is 20kV, and the laser frequency 1000Hz, laser energy 50%, the high-resolution mass spectrum of soybean oil sample is as follows figure 1 As shown, 1500 laser points are collected on each high-resolution mass spectrum collected by matrix-assisted laser desorption i...
Embodiment 2
[0058] This embodiment only describes the differences from the above-mentioned embodiments. In this embodiment, the high-resolution mass spectrometry data of triglyceride compounds in the standard sample are sequentially normalized and averaged, and partial least squares method-discriminant Analytical processing, constructing a lipidomics-based PLS-DA soybean and soybean oil origin traceability identification model. Such as image 3 As shown, the PLS-DA soybean and soybean oil origin traceability identification model can significantly distinguish Brazilian and non-Brazilian soybean samples, especially soybean samples from the United States, which are distributed throughout the non-Brazilian soybean area and clearly distinguished from the Brazilian soybean area .
Embodiment 3
[0060] This example only describes the differences from the above examples, in this example, the substrate is sinapinic acid or 2-cyano-4-hydroxycinnamic acid.
[0061] Example 1 of the Traceability Identification Model of Soybean and Soybean Oil Origin in Two Countries
[0062] This embodiment only describes the differences from the above-mentioned embodiments. In this embodiment, in the process of standard sample preparation in the step S1, it is assumed that the soybean samples come from n different regions, and the soybean samples are Divide into n×(n-1) / 2 groups, and each group of soybean samples consists of two soybean samples from different origins, and each group of soybean samples follows steps S2, S3, and S4 to establish the origin traceability of soybeans and soybean oil in the two countries The identification model is used to identify the country or region of soybean and soybean oil corresponding to the traceability identification model of soybean and soybean oil o...
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