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Method for evaluating microplastic pollution level based on plant physiology indexes

A pollution level, microplastic technology, applied in the field of environmental ecology, can solve the problem of difficult to know the impact of microplastics

Pending Publication Date: 2021-09-21
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since most of the current ecological risk assessments for microplastics are carried out based on marine animals, and the impact of microplastics on plants located at the border between land and sea such as Spartina alterniflora is difficult to know, there is an urgent need for a plant-based ecological risk assessment of microplastics. assessment method

Method used

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  • Method for evaluating microplastic pollution level based on plant physiology indexes
  • Method for evaluating microplastic pollution level based on plant physiology indexes
  • Method for evaluating microplastic pollution level based on plant physiology indexes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Seed collection: Collect Spartina alterniflora seeds that are plump and of uniform shape in the wild coastal wetlands.

[0031] (2) Microplastic treatment:

[0032] Collect the masks in the sediment, cut them into pieces, and pass through a 200-mesh sieve through a pulverizer to obtain microplastics, then clean the microplastics with ultrapure water and absolute ethanol, and then add HNO to the microplastics 3 +H 2 o 2 The mixed solution was soaked at room temperature for 1 h, then heated to 250 °C for 2 h, evaporated and concentrated after cooling down, and filtered with a 0.45 μm water phase filter head to obtain the microplastic sample to be used.

[0033] Microplastics of different weights were weighed, and ethanol and purified water (1:1) were added to them, followed by ultrasonication for 1 h to make them evenly distributed, and the concentrations of microplastics were 0, 0.2, 1, 2, 5, 10 , 50, 100 mg / kg suspension.

[0034] (3) Spartina alterniflora seeds...

Embodiment 2

[0037] (1) Seed collection: Collect Spartina alterniflora seeds that are plump and of uniform shape in the wild coastal wetlands.

[0038] (2) Microplastic treatment:

[0039] Collect the masks in the sediment, cut them into pieces, and pass through a 200-mesh sieve through a pulverizer to obtain microplastics, then clean the microplastics with ultrapure water and absolute ethanol, and then add HNO to the microplastics 3 +H 2 o 2 The mixed solution was soaked at room temperature for 1 h, then heated to 250 °C for 2 h, evaporated and concentrated after cooling down, and filtered with a 0.45 μm water phase filter head to obtain the microplastic sample to be used.

[0040] Microplastics of different weights were weighed, and ethanol and purified water (1:1) were added to them, followed by ultrasonication for 1 h to make them evenly distributed, and the concentrations of microplastics were 0, 0.2, 1, 2, 5, 10 , 50, 100 mg / kg suspension.

[0041] (3) Add Spartina alterniflora s...

Embodiment 3

[0045]The antioxidant system in plants has a certain response to pollution stress, which is the key factor determining the resistance of plant cells to oxidative stress, that is, under pollution stress, plants will produce oxygen free radicals during their internal metabolism, and excessive oxygen free radicals cause oxidative damage to plants. In this example, the levels of superoxide (POD), superoxide dismutation (SOD), catalase (CAT) and malondialdehyde (MDA) of Spartina alterniflora exposed to different concentrations of microplastic pollution were detected. To further explore the evaluation indicators of microplastic pollution levels.

[0046] The POD activity was determined by the guaiacol method, the SOD activity was determined by the NBT photoreduction method, the CAT activity was determined by the same method, and the MDA content was determined by thiobarbituric acid.

[0047] Plant roots are in direct contact with pollutants in soil / water and are the most sensitive ...

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Abstract

The invention provides a method for evaluating the microplastic pollution level based on plant physiology indexes, and belongs to the technical field of environmental ecology. According to the method, the method for evaluating the microplastic pollution level is established on the basis of the influence of micro-plastics on the growth and development of the spartina alterniflora, the result of the method is objective and real, and scientific theoretical support is provided for evaluating the growth and development and stress resistance response of the micro-plastics to plants in an ecological system.

Description

technical field [0001] The invention belongs to the technical field of environmental ecology, and in particular relates to a method for evaluating the pollution level of microplastics based on plant physiological indicators. Background technique [0002] With the progress of economy and the development of society, plastic products bring various potential risks while bringing convenience to people. Studies have shown that plastic products, such as discarded masks, will become microplastics that are not easily degraded by the natural environment after physical, chemical, and biological effects. Because this type of microplastic is too small to be removed from the ocean or watershed, it ends up causing great harm to marine life. [0003] The role of Spartina alterniflora in promoting siltation and land-building has had a positive impact on tidal flat construction, but due to its wide ecological breadth, it has invaded rapidly, and has now invaded many bays in the world. Since...

Claims

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Application Information

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IPC IPC(8): G01N33/50A01C1/02
CPCG01N33/5014G01N33/5097A01C1/025
Inventor 贾慧刘浩淼吴一凡李健
Owner JIANGSU UNIV
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