Detection method of content of neutralizing antibody of adeno-associated virus and construction method of cell line
A construction method and detection method technology, applied in the field of medical detection, can solve the problems of inability to meet the real-time monitoring of stable cell lines, long construction period, false positives of cell lines, etc., so as to avoid the problem of drug-resistant bacteria and shorten the construction time.
Pending Publication Date: 2021-09-24
SICHUAN UNIV
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The invention discloses a detection method of the content of a neutralizing antibody of an adeno-associated virus and a construction method of a cell line, and relates to the technical field of medical detection. The detection method comprises the following steps of: infecting cells with an overexpression vector of a miniAAVR-fluorescence reporter gene fusion gene, and overexpressing a miniAAVR-fluorescence reporter gene to obtain a cell line sensitive to adeno-associated virus infection. By utilizing the construction method provided by the invention, various animal cells can be quickly transformed into the cell line sensitive to the adeno-associated virus. As the method does not need repeated screening of antibiotics, the construction time of the cell line can be greatly shortened. Meanwhile, the fluorescence reporter gene is used based on the screening process, so that no antibiotic marker is used, false positive stable cell strains caused by drug resistance of the cells can be avoided, and the required stable cell line can be quickly screened.
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