Gingival epithelium model and in-vitro building method thereof
A construction method and gingival technology, applied in the field of tissue engineering biomaterials, can solve the problems of long construction time, poor model stratification, and reduced model application, and achieve the effects of reducing production cost, shortening construction time, and improving barrier function.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0026] Example 1: This example focuses on the steps of an in vitro construction method of a gingival epithelium model:
[0027] Step 1. Primary culture and expansion of human gingival epithelial cells:
[0028] Normal human gingival tissues were rinsed with 0.01M PBS containing 100IU / mL penicillin and 100μg / mL streptomycin under the condition of pH=7.4. Trim the tissue block, remove the subcutaneous connective tissue, and cut the tissue block into about 10mm 3 Size, wash with 0.01M PBS containing 100IU / mL penicillin and 100μg / mL streptomycin at pH=7.4, and digest with Dispase solution at 4℃ for 16h. Separate the tissue epithelial layer and subcutaneous connective tissue layer, transfer the separated epithelial layer into a centrifuge tube pre-installed with 1.5 mL 0.25% trypsin, incubate at 37°C for 5 minutes, and digest to obtain a single cell suspension. Add keratinocyte serum-free medium to resuspend the cells, transfer to a culture flask, change the medium after 12 hours, and ...
Example Embodiment
[0039] Example 2:
[0040] This example focuses on the steps of a method for constructing a gingival epithelium model in vitro:
[0041] Step 1. Primary culture and expansion of human gingival epithelial cells:
[0042] Normal human gingival tissues were rinsed with PBS 0.01M containing 100IU / mL penicillin and 100μg / mL streptomycin under a pH=7.4 environment. Trim the tissue block, remove the subcutaneous connective tissue, and cut the tissue block into about 10mm 3 For the size, wash with PBS 0.01M containing 100IU / mL penicillin and 100μg / mL streptomycin at pH=7.4, and digest with Dispase solution at 4℃ for 16h. Separate the tissue epithelial layer and subcutaneous connective tissue layer, transfer the separated epithelial layer into a centrifuge tube pre-installed with 1.5 mL 0.25% trypsin, incubate at 37°C for 5 minutes, and digest to obtain a single cell suspension. Add keratinocyte serum-free medium to resuspend the cells, transfer to a culture flask, change the medium after 1...
Example Embodiment
[0053] Example 3:
[0054] This example focuses on the steps of a method for constructing a gingival epithelium model in vitro:
[0055] Step 1. Primary culture and expansion of human gingival epithelial cells:
[0056] Normal human gingival tissues were rinsed with PBS 0.01M containing 100IU / mL penicillin and 100μg / mL streptomycin under a pH=7.4 environment. Trim the tissue block, remove the subcutaneous connective tissue, and cut the tissue block into about 10mm 3 For size, wash with PBS 0.01M containing 100IU / mL penicillin and 100μg / mL streptomycin at pH=7.4, and digest with Dispase solution at 4℃ for 16h. Separate the tissue epithelial layer and subcutaneous connective tissue layer, transfer the separated epithelial layer into a centrifuge tube pre-installed with 1.5 mL 0.25% trypsin, incubate at 37°C for 5 minutes, and digest to obtain a single cell suspension. Add keratinocyte serum-free medium to resuspend the cells, transfer to a culture flask, change the medium after 12 ho...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap