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Chemotactic factor as molecular marker for diagnosing rosacea

A chemokine, rosacea technology, applied in the field of biomedicine, can solve problems such as unclear rosacea

Active Publication Date: 2021-10-01
THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, studies have shown that TLR2 is highly expressed in skin lesions of patients with rosacea, but whether TLR2 promotes the pathogenesis of rosacea by up-regulating the expression of the above chemokines is still unclear

Method used

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  • Chemotactic factor as molecular marker for diagnosing rosacea
  • Chemotactic factor as molecular marker for diagnosing rosacea
  • Chemotactic factor as molecular marker for diagnosing rosacea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 mouse experiment.

[0028] Female Balb / c mice aged 6-8 weeks were selected and randomly divided into a control group, a model group and a treatment group. The control group received no treatment, and the model group was subcutaneously injected with 40 μL LL-37 (320 μM) on the back to create animal models of rosacea, 12 hours each time, a total of 4 times; the treatment group was stimulated with 0.5% AhR 30 minutes after the second injection of LL-37. Benvimod treatment was given for 3 consecutive days; photographs were taken 24 hours after the last dose to evaluate the erythema. The changes under the microscope were observed by HE staining, and the levels of TLR2 and chemokines CCL5, CXCL9, CXCL10, and CXCL11 were detected by qRT-PCR.

[0029] 1. Erythema scoring method: Score 1-5 according to the severity of erythema (5 is the highest); use Image J software to measure the erythema area of ​​skin lesions taken in digital photos, and conduct quantitative an...

Embodiment 2

[0069] Example 2 Cell experiment.

[0070] Add LL-37 to HaCaT cells to create a rosacea cell model, and then add the AhR agonist Benvimod to treat it. The levels of TLR2 and chemokines CCL5, CXCL9, CXCL10 and CXCL11 were detected by qRT-PCR and Western Blot / ELISA; the levels of chemokines CCL5, CXCL9, CXCL10 and CXCL11 were detected by lentivirus transfection to overexpress TLR2.

[0071] 1. Cell culture:

[0072] HaCaT cells were cultured in DMEM medium containing 10% FBS and 1% penicillin and streptomycin, and placed in 5% CO 2 in a 37 °C humidified incubator. Replace the culture medium once every 1-2 days, observe the growth of the cells under a microscope, and pass the cells when the coverage rate reaches 80-90%. To operate in the cell cleaning workbench, first use a pipette to aspirate the liquid in the bottle / dish, and wash the cells with 2 mL of sterile PBS for a total of 2 washes. Then add trypsin 1-2mL to the culture flask, 37°C, 5% CO 2 Incubate in the incubator...

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Abstract

The invention belongs to the field of biological medicine, and particularly relates to the application of a chemotactic factor serving as a rosacea related molecular marker in diagnosis and treatment of the rosacea related molecular marker. The expression of TLR2 and chemotactic factors CCL5, CXCL9, CXCL10 and CXCL11 in rosacea animals induced by LL-37 is increased, and the AhR agonist benvimod can relieve erythema and inflammatory cell infiltration and reduce the expression of the TLR2 and the chemotactic factors. The expression of TLR2 and chemotactic factors CCL5, CXCL9, CXCL10 and CXCL11 in a rosacea cell model induced by LL-37 is increased, and the expression of TLR2 and the chemotactic factors can be reduced by an AhR agonist, namely, benvimod; the up-regulation of the TLR2 can cause the increase of the expression of the chemotactic factor in the cell model, and the AhR agonist benvimod can reduce the expression of the chemotactic factor by down-regulating the expression of the TLR2. The chemotactic factors CCL5, CXCL9, CXCL10 and CXCL11 are expected to become new diagnosis indexes or treatment targets of rosacea diseases.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to the application of a chemokine as a rosacea-related molecular marker in its diagnosis and treatment. Background technique [0002] Chemokines are secreted proteins with small molecular weight (8-14kd), have chemotactic activity, and play an important role in the maintenance of immune homeostasis and the process of inflammatory response. Chemokines are divided into four subtypes according to their structure, including CC, CXC, C and CX3C, which exert biological effects by interacting with homologous cell surface chemokine receptors. About 50 chemokines have been discovered so far, mainly involved in the development, differentiation and migration of leukocytes, and also play an important role in inflammatory diseases, angiogenesis and wound repair. [0003] Aryl hydrocarbon receptors (AhR) can combine with polycyclic aromatic hydrocarbons and dioxins in the environment, and c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883G01N33/68G01N33/577A61K45/00A61P17/10
CPCC12Q1/6883G01N33/6863G01N33/6893G01N33/577A61K45/00A61P17/10C12Q2600/158C12Q2600/136G01N2333/522G01N2333/523G01N2800/20G01N2800/7095
Inventor 孙艳吴严陈良宏乔帅肖碧环王敬玉褚海涛程铭高兴华
Owner THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV
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