Electrochemical sensor for detecting ascorbic acid, uric acid and dopamine and preparation method of electrochemical sensor

Abstract

The invention provides an electrochemical sensor for detecting ascorbic acid, uric acid and dopamine and a preparation method of the electrochemical sensor, and belongs to the technical field of electrochemical sensors. The preparation method of the electrochemical sensor comprises the following steps of: preparing zinc oxide nano-particles by adopting a hydrothermal method; forming a zinc oxide nano-particle dispersion liquid; modifying a glassy carbon electrode by utilizing the zinc oxide nano-particle dispersion liquid; and covering the surface of the glassy carbon electrode with a layer of perfluorosulfonic acid type polymer solution, so as to obtain the electrochemical sensor. The electrochemical sensor capable of simultaneously determining the ascorbic acid (AA), dopamine (DA) and uric acid (UA) is prepared by adopting the ZnONPs modified glassy carbon electrode (GCE). Three analyte oxidation peaks can be successfully separated out by utilizing the electrochemical sensor, so that ascorbic acid, uric acid and dopamine can be simultaneously detected; and the electrochemical sensor has good detection performance on the three analytes and has potential clinical application value.

Description

technical field [0001] The invention belongs to the technical field of electrochemical sensors, and in particular relates to a preparation method of an electrochemical sensor for detecting ascorbic acid, uric acid and dopamine, and an electrochemical sensor for detecting ascorbic acid, uric acid and dopamine. Background technique [0002] Ascorbic acid (AA), dopamine (DA) and uric acid (UA) are active substances with important biological research value existing in the extracellular fluid of the human central nervous system. Among them, ascorbic acid (AA) plays an important role in promoting biological growth and synthesizing antibodies, and dopamine (DA) is an important biological small molecule substance in the human central nervous system. Cholesterol below or exceeding normal levels in the human body will directly affect the mental activity of the human body. When the purine metabolism in the human body is abnormal, excess uric acid (UA) will be produced, and the remaini...

AI Technical Summary

Problems solved by technology

Enzymatic detection of uric acid is relatively simple, but the high cost of the enzyme and the constant temperature of the reaction process limit its application. The recognition specificity between the enzyme and the substrate makes the enzyme sensor have high selectivity and low detection limit.

However, the activity of enzymes is greatly affected by external environmental factors such as pH, temperature, and material toxicity, which makes enzyme sensors have low stability and short lifespan.

At the same time, due to the limited types of enzymes, their application range is greatly limited.

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