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Application of GYY4137 in preparation of drugs for inhibiting pyroptosis of vascular endothelial cells

A 1.GYY4137, vascular endothelium technology, applied in the field of biomedicine, can solve problems such as aggravating vascular inflammation and damage to arterial structure and function

Inactive Publication Date: 2021-10-08
SHIHEZI UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, activated IL-1β and IL-18 can also be released through GSDMD-N membrane perforation to aggravate vascular inflammation, and eventually lead to damage to arterial structure and function and the occurrence and development of the above-mentioned cardiovascular diseases

Method used

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  • Application of GYY4137 in preparation of drugs for inhibiting pyroptosis of vascular endothelial cells
  • Application of GYY4137 in preparation of drugs for inhibiting pyroptosis of vascular endothelial cells
  • Application of GYY4137 in preparation of drugs for inhibiting pyroptosis of vascular endothelial cells

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Embodiment 1

[0037] 1 material

[0038] 1.1 Cell Human umbilical vein endothelial cells (Human umbilical vein endothelial cells, HUVECs) were purchased from American Type Culture Collection (ATCC) laboratory.

[0039] 1.2 Drugs and reagents Special culture medium for endothelial cells and fetal bovine serum were purchased from Sciencell, USA; oxidized low-density lipoprotein (oxLDL) was purchased from Guangzhou Yiyuan Biotechnology Co., Ltd. Homocysteine ​​(Hcy), lipopolysaccharide (LPS ), H 2 S donor GYY4137 and endogenous H 2 S synthase inhibitor DL-propargylglycine (D,L-propargylglycine, PAG) was purchased from Sigma-Aldrich, USA; CCK-8 cell viability detection kit was purchased from Dongren Chemical Technology (Shanghai) Co., Ltd.; Hoechst33342 / Propidium iodide (PI) double staining kit and lactate dehydrogenase (lactate dehydrogenase, LDH) cytotoxicity detection kit were purchased from Nanjing Jiancheng Bioengineering Institute; Caspase-1 activity detection kit was purchased from Sh...

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Abstract

The invention provides application of GYY4137 in preparation of drugs for inhibiting pyroptosis of vascular endothelial cells, and belongs to the technical field of biological medicines. The H2S donor GYY4137 can be used for inhibiting expression up-regulation (P is less than 0.05 and P is less than 0.01) of vascular endothelial cell pyroptosis key proteins (NLRP3, Pro-Caspase-1, Caspase-1 (P20), GSDMD, GSDMD-N and IL-18) induced by cardiovascular disease pathogenic risk factors, namely oxidized low-density lipoprotein (oxLDL) and homocysteine (Hcy), and inhibiting the activity of a vascular endothelial cell pyroptosis key enzyme Caspase-1 by down-regulating an NLRP3 inflammasome-Caspase-1 signal channel, and therefore, pyroptosis of the vascular endothelial cells is inhibited.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of GYY4137 in the preparation of drugs for inhibiting pyroptosis of vascular endothelial cells. Background technique [0002] Pyroptosis is a new programmed and inflammatory cell death mode discovered after apoptosis and necrosis. In recent years, studies have shown that inflammatory death of vascular endothelial cells—cell pyroptosis plays an important role in many cardiovascular diseases (hyperlipidemia, hypertension, atherosclerosis, coronary heart disease, heart failure), metabolic diseases (diabetes and its complications such as Retinopathy) and the development of chronic inflammatory diseases play a key role. [0003] The key molecular pathway of pyroptosis is nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) / cysteine-containing aspartic acid proteolytic enzyme- 1 (cysteinyl aspartate specific proteinase 1, Caspase-1) mediated c...

Claims

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Application Information

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IPC IPC(8): A61K31/675A61P43/00A61P9/00A61P3/00A61P29/00
CPCA61K31/675A61K9/0019A61P43/00A61P9/00A61P3/00A61P29/00
Inventor 张亮李玲何婷婷张旭琳
Owner SHIHEZI UNIVERSITY
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