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A kind of Prunella vulgaris extract with anti-hsv activity and its preparation method and medical use

A technology of Prunella vulgaris and extracts, which is applied in the field of medicine, can solve the problems of drug resistance and other problems, and achieve the effect of stabilizing anti-HSV, inhibiting paralysis of hind limbs, and reducing symptoms of vaginal infection

Active Publication Date: 2022-07-01
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Clinical treatment drugs are mainly nucleoside analogues such as acyclovir (ACV). For severe and frequent relapse cases, large doses of long-term administration are required, which is prone to drug resistance

Method used

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  • A kind of Prunella vulgaris extract with anti-hsv activity and its preparation method and medical use
  • A kind of Prunella vulgaris extract with anti-hsv activity and its preparation method and medical use
  • A kind of Prunella vulgaris extract with anti-hsv activity and its preparation method and medical use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The technical scheme of the present invention is carried out according to the following steps:

[0042] The preparation method of different extraction parts of Prunella vulgaris comprises the following steps:

[0043] (1) Preparation of different solvent extracts of Prunella vulgaris (see figure 1 )

[0044] Prunella vulgaris 90% ethanol extract: take 50 g of Prunella vulgaris, add 20 times the volume and mass percentage concentration of ethanol to extract twice, 2 hours, filter, discard the medicinal residues, concentrate the extract, 50 ℃ rotary evaporation under reduced pressure concentration, After drying, 90% ethanol extract of Prunella vulgaris was obtained, named PV90E.

[0045] Prunella vulgaris 70% ethanol extract: take 50 g of Prunella vulgaris, add 20 times the volume and mass percentage concentration of 70% ethanol to extract twice, 2 hours, filter, discard the medicinal residues, concentrate the extract, concentrate under reduced pressure by rotary evapor...

Embodiment 2

[0055] In this example, the identification reaction, HPGPC and the fractions of graded alcohol precipitation (PVE30, PVE50, PVE70, PVE85) were used for chemical characterization, and the alcohol extracts of Prunella vulgaris (PV90E, PV70E) and the water-extracted alcohol precipitation supernatant (PVES) were used for chemical characterization. UPLC-DAD-QTOF-MS / MS analysis was performed.

[0056] (1) Identification reaction of water extract, alcohol extract and fractional alcohol precipitation of Prunella vulgaris

[0057] Methods: Molish reaction (saccharides), iodine solution reaction (starch), Coomassie brilliant blue reaction (protein), ninhydrin reaction (free amino acid), FeCl reaction were carried out on the above parts. 3 Reaction (tannin), acetic anhydride concentrated sulfuric acid reaction (saponins), Feigl reaction (quinones), magnesium hydrochloride powder reaction (flavonoids), potassium iodide precipitation reaction (alkaloids), etc.

[0058] Results: Molish rea...

Embodiment 3

[0087] The anti-HSV-1 / KOS and HSV-2 / G standard strains were tested for the activity of each extracted part of Prunella vulgaris prepared in Example 1, and the part with the best anti-HSV activity was screened out.

[0088] Methods: The in vitro inhibitory effect of Prunella vulgaris extract on standard virus strains HSV-1 / KOS and HSV-2 / G was detected by CCK-8 colorimetric method.

[0089] After the Vero cells were counted, the cells were seeded in a 96-well cell culture plate at a density of 20,000 cells per well, and placed in a constant temperature of 37°C, CO. 2 In an incubator with a concentration of 5% by volume, after the cells have grown to a monolayer, discard the supernatant medium, add 100×TCID 50 After one hour of adsorption, different concentrations of Prunella vulgaris extract samples (100 μg / mL, 50 μg / mL, 25 μg / mL, 12.5 μg / mL) were added, each concentration was 3 duplicate wells, 200 μL / well, at 37 ℃, volume percentage 5%CO 2 Culture for 3 days, add CCK-8 after...

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Abstract

A Prunella vulgaris extract with anti-HSV activity, taking Prunella vulgaris ear or powder thereof, adding 10-22 volume times of water, heating and refluxing for extraction 2-4 times, filtering, combining filtrates, concentrating under reduced pressure, and then adding 0.2- 1 volume of ethanol so that the ethanol concentration is 10-50% v / v, and centrifugation after standing at 4°C for 6-24 hours, the obtained precipitate is washed at least twice with 10-50% v / v ethanol, and the precipitate is collected, Ready to dry. The present invention also provides the above-mentioned preparation method and application of the Prunella vulgaris extract of the present invention. The Prunella vulgaris extract provided by the invention has significant anti-HSV effect in vitro and in vivo, its anti-HSV activity in vitro is doubled compared with that of the water extract, and also has a stable inhibitory effect on ACV-resistant HSV clinical strains and drug-resistant strains . At the same time, it has good curative effect on the skin and genital herpes caused by HSV-1 and HSV-2 infection.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a Prunella vulgaris, in particular to a Prunella vulgaris extract with anti-HSV activity, a preparation method and medicinal uses thereof. Background technique [0002] Herpes simplex virus (HSV) is divided into type I (HSV-1) and type II (HSV-2). The virus invades the body through the oral cavity and genitals, and has long-term latent and recurrent attacks. disease and serious complications. The clinical treatment drugs are mainly nucleoside analogs such as acyclovir (ACV). Therefore, there is an urgent need to develop anti-HSV drugs with different structures and mechanisms of action from acyclovir. [0003] The applicant team established an antiviral screening platform for traditional Chinese medicines in the early stage, and systematically screened traditional Chinese medicines with heat-clearing and detoxifying effects and antiviral activities reported in the literature. ef...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/175A61K36/536A61P31/22A23L33/105A61K131/00
CPCA61K36/536A61P31/22A23L33/105A61K2236/331A61K2236/39A61K2236/53A61K2236/51A23V2002/00A23V2200/30A23V2250/21
Inventor 徐宏喜张群铄李洋付文卫钟煊蕾冯极灵罗小妹袁满
Owner SHANGHAI UNIV OF T C M
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