Entomopathogenic nematode symbiotic bacterium with broad-spectrum antibacterial function and application thereof

An entomopathogenic nematode, broad-spectrum antibacterial technology, applied in the field of microorganisms, can solve problems such as no reports of biological pesticides

Active Publication Date: 2021-10-08
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the antibacterial compounds of this bacterium are mainly used in medicine, and there are no reports of their development as biological pesticides.

Method used

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  • Entomopathogenic nematode symbiotic bacterium with broad-spectrum antibacterial function and application thereof
  • Entomopathogenic nematode symbiotic bacterium with broad-spectrum antibacterial function and application thereof
  • Entomopathogenic nematode symbiotic bacterium with broad-spectrum antibacterial function and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Types identification

[0018] XBD102 is a combination of bihedezi pathogens from Slim Strigen in the Soil Soil Sites collected from Liaoning Province, and the 16S RRNAGENE of the strain was identified by strains, and the PCR amplification primers used were:

[0019] Upstream Primers: 27F: 5'-agagttgatcctggctcAg-3 ';

[0020] Downstream primers: 1492R: 5'-ggttaccttgttacgactt-3 ';

[0021] The carrier is constructed and converted to E. coli, and the 16S RDNA sequence comparison is performed. figure 1 As shown, from figure 1 It can be seen that the present embodiment is separated by a XenorhabDus BudapeStensis, named XBD102.

Embodiment 2

[0022] Example 2: Determination of XBD102 Fermentation Liquid Antibacterial Activity

[0023] Use NBTA agar medium (trypsin 10g / L; beef powder 3g / L; sodium chloride 5g / L; chlorinated triphenylttiazole 0.04 g / L; bromide phenol deflast 0.025 g / L, pH = 7.8 The XBD102 scrubbing, 28 ° C for 48 hours, and blue brown light colonies can be seen. Picking the single collapse falls to the LB medium (trypsin 10g / L; yeast extract 5g / L; sodium chloride 10g / L), 28 ° C; 200 rpm culture 14 hours to form fermentation seed liquid, 2% bacterial The seed fluid is attached to a triangular bottle containing an LB liquid medium, fermented 48 hours with the above conditions, and dispelled with an ankle from high speed, and the sterilization of sterile filters (0.22 μm) is filtered. The sterile fermentation supernatant is Antibacterial test to be tested.

[0024] 1. Identification of the inhibitory activity of fungi

[0025] 1 ml of sterile fermented supernatant was mixed with 9 ml of melte...

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Abstract

The invention relates to an entomopathogenic nematode symbiotic bacterium XBD102 (Xenorhabdus budapestensis) strain with a broad-spectrum antibacterial function, and the entomopathogenic nematode symbiotic bacterium XBD102 strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No: 22057. A fermentation supernatant of the XBD102 strain has a growth inhibition effect on various fungi and bacteria, and can be used for development and application of novel biological pesticide.

Description

Technical field [0001] The present invention relates to the field of microbiological technology, and in particular, there is a variety of insect diseases with broad spectrum bacteria function and its application. Background technique [0002] Insect disease, the original bacterium is a Gram-negative bacterium existing in the intestinal tract of the insect disease, which is affiliated with deformation bacteria PROTEOBACTERIA's γ-sub-sub-plane, including the pathogens and Portorhabdus, two bacteria, They are symbiotic symbiosis with the pathogens of the Schwheworm and the isotrimer infection period (Forst et al., 1997): The pathogenic straps infect insects, release the common bacteria to the insect body cavity, and propagate and produce toxins. And hydrolyzable extracellular enzymes, eventually leading to the death of the host insects. [0003] Studies have shown that insect disease-inducereps, but also produce a variety of antibacterial compounds, but also produces a variety of an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/20A01P1/00A01P3/00C12R1/01
CPCC12N1/20A01N63/20
Inventor 李广悦李琲琲杨秀芬任杰曾洪梅袁京京
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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