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Magnetic bead for efficiently adsorbing extracellular DNA in environment, and preparation method thereof

A magnetic bead and high-efficiency technology, which is applied in the field of magnetic beads and its preparation for high-efficiency adsorption of extracellular DNA in an environment, can solve the problem of insufficient adsorption specificity and achieve high specificity

Active Publication Date: 2021-10-22
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The adsorption specificity of the magnetic beads to DNA is not high enough

Method used

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  • Magnetic bead for efficiently adsorbing extracellular DNA in environment, and preparation method thereof
  • Magnetic bead for efficiently adsorbing extracellular DNA in environment, and preparation method thereof
  • Magnetic bead for efficiently adsorbing extracellular DNA in environment, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1) Take 4g nFe 3 o 4 (particle size of 15nm) was dissolved in 80mL of water, 8g of tetraethyl orthosilicate (TEOS) and 75g of glycerin were added, and the pH of the mixture was adjusted to 4.2 with glacial acetic acid. The mixture was then stirred and heated at 90°C for 2 hours. After the reaction is complete, the product is cooled and separated from the solution by a magnet. The separated solids were washed with water and ethanol, respectively, to obtain clean supported SiO 2 Fe 3 o 4 Nanoparticles (SiO 2 @nFe 3 o 4 ).

[0022] 2) 1g of SiO prepared in the above steps 2 @nFe 3 o 4 Disperse in 200mL ethanol solution, add 1g CTAB, and continuously stir and reflux at 80°C for 12h. The solid material was recovered with a magnet and washed with water and ethanol, respectively. The sample was then dispersed in 100 mL of an ethanol solution containing 1 g of ammonium nitrate and refluxed at 80° C. for 24 hours. The solid matter was recovered by a magnet and wash...

Embodiment 2

[0025] 1) Take 2g nFe 3 o 4(Particle size is 30nm) was dissolved in 100mL of water, and then 2g of tetraethyl orthosilicate (TEOS) and 20g of glycerin were added, and the pH of the mixture was adjusted to 5.0 with glacial boric acid. The mixture was then stirred and heated at 70°C for 1 hour. After the reaction is complete, the product is cooled and separated from the solution by a magnet. The separated solids were washed with water and ethanol, respectively, to obtain clean supported SiO 2 Fe 3 o 4 Nanoparticles (SiO 2 @nFe 3 o 4 ).

[0026] 2) 2g of the SiO prepared by the above steps 2 @nFe 3 o 4 Disperse in 200mL methanol solution, add 1g P123, and continuously stir and reflux at 60°C for 6h. The solid material was recovered with a magnet and washed with water and ethanol, respectively. The sample was then dispersed in 100 mL of methanol containing 1 g of ammonium nitrate and refluxed at 60° C. for 12 hours. The solid matter was recovered by a magnet and wash...

Embodiment 3

[0029] 1) Take 5g nFe 3 o 4 (particle size of 25nm) was dissolved in 200mL of water, and then 20g of tetraethyl orthosilicate (TEOS) and 200g of glycerol were added, and the pH of the mixture was adjusted to 3.0 with glacial acetic acid. The mixture was then stirred and heated at 95°C for 3 hours. After the reaction is complete, the product is cooled and separated from the solution by a magnet. The separated solids were washed with water and ethanol, respectively, to obtain clean supported SiO 2 Fe 3 o 4 Nanoparticles (SiO 2 @nFe 3 o 4 ).

[0030] 2) 3g of SiO obtained in the above steps 2 @nFe 3 o 4 Disperse in 300mL ethanol solution, add 6g CTAB, and continuously stir and reflux at 90°C for 12h. The solid material was recovered with a magnet and washed with water and ethanol, respectively. The sample was then dispersed in 100 mL of ethanol containing 6 g of ammonium nitrate and refluxed at 90° C. for 36 hours. The solid matter was recovered by a magnet and wash...

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Abstract

The invention relates to a magnetic bead for efficiently adsorbing extracellular DNA in an environment, and a preparation method thereof. The magnetic bead is of a nano spherical double-layer structure, the particle size is 15-30 nm, the specific surface area is 65-210 m<2> / g, the inner layer is magnetic nano ferroferric oxide (nFe3O4), the outer layer is a mesoporous-loaded silane coating layer, and the magnetic bead contains hydroxyl functional groups. Firstly, nano ferroferric oxide (nFe3O4) magnetic beads are wrapped with silicon dioxide, then a coating layer is formed again by using a surfactant, the magnetic beads capable of efficiently adsorbing extracellular DNA in the environment are obtained after the surfactant is eluted, and the magnetic beads have the characteristics of small particle size and large specific surface area, can highly selectively adsorb extracellular DNA in the environment in the presence of pollutants, and can be used for efficiently adsorbing the extracellular DNA in the environment. The adsorption time is short, the adsorption capacity is large, and the method can be used for efficiently extracting extracellular DNA in the environment.

Description

technical field [0001] The invention relates to a magnetic bead for efficiently adsorbing extracellular DNA in the environment and a preparation method thereof. The magnetic bead has the characteristics of small particle size and large specific surface area, and can highly selectively adsorb cells in the environment in the presence of pollutants. Extracellular DNA, with short adsorption time and large adsorption capacity, can be used for efficient extraction of extracellular DNA in the environment. Background technique [0002] Extracellular DNA is an important part of DNA in the environment. It can exist independently of cells for a long time and contains rich genetic information. For example, in archaeology, extracellular DNA is often used to discover ancient biological information. In addition, extracellular DNA is also used to explore plants. The interconnection with pollinators, the analysis and detection of food chains and the identification of alien species invasion, ...

Claims

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Application Information

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IPC IPC(8): B01J20/06B01J20/10B01J20/28B01J20/30C12N15/10
CPCB01J20/06B01J20/103B01J20/28009B01J20/28059B01J20/28061C12N15/1013
Inventor 袁青彬梁张岐王尚杰程远林群超
Owner NANJING UNIV OF TECH
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