Method for enhancing production of medium-chain fatty acid by anaerobic fermentation of residual sludge
A medium-chain fatty acid, anaerobic fermentation technology, applied in the field of environmental engineering by using magnetite to improve the production of medium-chain fatty acids from urban surplus activated sludge
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Embodiment 1
[0028] (1) Schematic diagram of the reactor structure for anaerobic fermentation to produce medium-chain fatty acids figure 1 As shown, the effective volume is 500mL, the mixing method is mechanical stirring, and the temperature control mode is heating in a water bath. Add 200 g of pyrolysis sludge fermentation liquid into the reactor, and add 200 g of anaerobic digestion sludge;
[0029] (2) Add the ethanol of 240mM in the anaerobic reactor, add the sodium 2-bromoethylsulfonate (methane inhibitor) of 5g / L;
[0030] (3) Using 2M hydrochloric acid solution, the initial pH of the reactor was adjusted to 6.5;
[0031] (4) Add 5g / L, 200-mesh magnetite powder (the main component is ferroferric oxide) into the reactor, blow off the air in the reactor with nitrogen for 10min, and seal the air collecting bag to ensure the system strict anaerobic state;
[0032] (5) The fermentation reaction temperature is 35°C, the rotating speed of the mechanical stirring paddle is 150rpm / min, and...
Embodiment 2
[0035] (1) Add 200 g of pyrolysis sludge fermentation liquid to the anaerobic fermentation reactor, and add 200 g of anaerobic digestion sludge;
[0036] (2) Add the ethanol of 240mM in the anaerobic reactor, add the sodium 2-bromoethylsulfonate (methane inhibitor) of 5g / L;
[0037] (3) Using 2M hydrochloric acid solution, the initial pH of the reactor was adjusted to 6.5;
[0038] (4) Add 10g / L, 200-mesh magnetite powder (the main component is ferroferric oxide) into the reactor, blow off the air in the reactor with nitrogen for 10min, and seal the air collecting bag to ensure that the system strict anaerobic state;
[0039] (5) The fermentation reaction temperature is 35°C, the rotating speed of the mechanical stirring paddle is 150rpm / min, and the sequential batch feeding and discharging mode is adopted. After 20 days, the VFAs and MCFAs in the fermentation broth are determined by gas chromatography, wherein hexanoic acid is MCFAs The main component of COD, with a concent...
Embodiment 3
[0042] (1) Add 200 g of pyrolysis sludge fermentation liquid to the anaerobic fermentation reactor, and add 200 g of anaerobic digestion sludge;
[0043] (2) Add the ethanol of 240mM in the anaerobic reactor, add the sodium 2-bromoethylsulfonate (methane inhibitor) of 5g / L;
[0044] (3) Using 2M hydrochloric acid solution, the initial pH of the reactor was adjusted to 6.5;
[0045] (4) Add 20g / L, 200-mesh magnetite powder (the main component is ferroferric oxide) into the reactor, blow off the air in the reactor with nitrogen for 10min, and seal and connect the air bag to ensure the system strict anaerobic state;
[0046] (5) The fermentation reaction temperature is 35°C, the rotating speed of the mechanical stirring paddle is 150rpm / min, and the sequential batch feeding and discharging mode is adopted. After 20 days, the VFAs and MCFAs in the fermentation broth are determined by gas chromatography, wherein hexanoic acid is MCFAs The main component is 13.28g COD / L, accountin...
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