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System for directionally converting dipeptide through continuous flow membrane enzyme coupling

A directional transformation, flow membrane enzyme technology, applied in the direction of supporting/immobilizing microorganisms, specific-purpose bioreactors/fermenters, biochemical instruments, etc., to increase contact time, increase contact area, and improve conversion rate and yield Effect

Inactive Publication Date: 2021-11-02
ANHUI ANLITAI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on the continuous flow membrane enzyme-coupled directional conversion system of dipeptides. Further research is still needed to improve the conversion rate and yield of dipeptide products by continuously reacting unreacted amino acid raw materials with biological enzymes.

Method used

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  • System for directionally converting dipeptide through continuous flow membrane enzyme coupling
  • System for directionally converting dipeptide through continuous flow membrane enzyme coupling
  • System for directionally converting dipeptide through continuous flow membrane enzyme coupling

Examples

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Embodiment 1

[0023] Such as figure 1 As shown, this embodiment provides a system for continuous flow membrane enzyme coupled directional conversion dipeptide, including membrane enzyme coupled reactor 100, first stirred tank 200, second stirred tank 300, biological enzyme tank 400, membrane enzyme coupled reaction The device 100 is formed by a plurality of membrane-enzyme coupled reaction units 110 equidistantly arranged from top to bottom. The first stirred tank 200 and the second stirred tank 300 are respectively filled with different amino acid raw materials. The first stirred tank 200 and the second stirred tank The amino acid raw materials in the stirring tank 300 enter from one end of the bottommost membrane enzyme coupling reaction unit 110 after being mixed, and the biological enzyme liquid in the biological enzyme tank 400 enters from the other end of the bottommost membrane enzyme coupling reaction unit 110; One end of the enzyme-coupled reaction unit 110 is connected to a liquid...

Embodiment 2

[0037] Such as Figure 1-4 As shown, this embodiment provides a method for continuous flow membrane enzyme-coupled directed conversion of dipeptides, comprising the following steps:

[0038] a. The amino acid raw materials in the first stirring tank 200 and the second stirring tank 300 are mixed through the first feeding pipe 270 and the second feeding pipe 280 respectively, and the flow rate is adjusted by the flow regulator 290 under the peristaltic action of the first peristaltic pump 291 Enter the membrane enzyme coupling reaction unit 110 at the bottom, and the biological enzyme liquid enters the membrane enzyme coupling reaction unit 110 at the bottom under the peristaltic action of the second peristaltic pump 420;

[0039] b. Amino acid raw material and biological enzyme liquid are mixed in the reaction tank body 111, enter the filtration through the diaphragm mesh layer 117 and the nanofiltration membrane layer 118 from the sealing material frame 115, and keep the pres...

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Abstract

The invention provides a system for directionally converting dipeptide through continuous flow membrane enzyme coupling, and relates to the technical field of continuous flow membrane enzyme coupling. According to the system for directionally converting the dipeptide through continuous flow membrane enzyme coupling, amino acid raw materials in the first stirring tank and the second stirring tank are mixed and then conveyed from the bottommost membrane enzyme coupling reaction unit, and biological enzyme liquid in the biological enzyme tank is conveyed from the other end of the bottommost membrane enzyme coupling reaction unit, so a continuous flow mixing reaction of the amino acid and the biological enzyme is formed; the plurality of membrane enzyme coupling reaction units which are arranged up and down form a long channel, so the contact area and the contact time among raw materials are increased, and a product is intercepted by a nanofiltration membrane in the membrane enzyme coupling reaction unit in time; the incompletely reacted amino acid raw material and the biological enzyme are circulated through the liquid storage tank and the return pipe and then enter the membrane enzyme coupling reaction unit for continuous reaction until the completely converted dipeptide is completely intercepted, so the conversion rate and the yield of the dipeptide product are improved.

Description

technical field [0001] The invention relates to the technical field of continuous flow membrane enzyme coupling, in particular to a continuous flow membrane enzyme coupling directional conversion dipeptide system. Background technique [0002] In order to solve the above shortcomings of the traditional enzymatic hydrolysis reaction, many scholars have begun to study the enzymatic hydrolysis-membrane separation coupling reaction technology (referred to as "enzyme-membrane coupling technology") in recent years, that is, the reactor and the separation membrane are connected by means of a circulating pump. At the same time as the enzymatic hydrolysis reaction, the enzymatic hydrolyzate that has reached the molecular weight is separated from the reaction system in time by membrane separation, and at the same time, the reaction substrate is continuously replenished at a certain speed until the remaining reaction residue in the reaction system seriously blocks the separation membran...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/40C12M1/36C12M1/34C12M1/12C12M1/02C12M1/00
Inventor 张洪斌马云峰周革
Owner ANHUI ANLITAI BIOTECH CO LTD
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