Trichoderma asperellum strain and application thereof

A technology of Trichoderma aculeatus and Trichoderma, applied in the direction of application, fungicides, fungi, etc., can solve problems affecting the quality and safety of tea products and the ecological safety of tea gardens, so as to improve disease resistance, increase tea green production, and improve tea production. The effect of quality ingredients

Active Publication Date: 2021-11-05
GUIZHOU TEA RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For a long time, chemical pesticides have been an important plant protection measure in the tea planting industry. However, as the occurrence of tea garden diseases has become more and more serious, the frequency of pesticide use has also gradually increased, which has led to increasingly prominent problems such as 3R, which seriously affects the quality and safety of tea products. Tea Garden Ecological Security

Method used

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  • Trichoderma asperellum strain and application thereof
  • Trichoderma asperellum strain and application thereof
  • Trichoderma asperellum strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] This embodiment is the isolation, screening, identification and preservation of Trichoderma asperellum SX-KXTM-T11 Trichoderma asperellum SX-KXTM-T11:

[0020] 1. Isolation of Trichoderma from tea tree rhizosphere:

[0021] After removing the surface soil and rotting leaves of the root circle of the tea tree with a shovel, the soil with a depth of about 10 cm was excavated by the five-point sampling method, put into a sterile sample bag and brought back to the laboratory, air-dried and set aside. Trichoderma strains were isolated from tea rhizosphere soil samples by gradient dilution method. Weigh 5g of air-dried soil sample and pour it into an Erlenmeyer flask filled with 45mL of sterile water, shake and incubate at 28°C and 150r / min for 30min, and mix thoroughly to obtain a concentration of 10 -1 g / mL sample suspension. Dilute it one by one to 10 in a clean bench -2 g / mL, 10 -3 g / mL and 10 -4 g / mL concentration, take 10 with a pipette gun after standing -3 g / mL ...

Embodiment 2

[0042] Present embodiment is that Trichoderma asperellum SX-KXTM-T11 Trichoderma asperellum SX-KXTM-T11 fermented liquid improves the disease resistance of tea tree:

[0043] The test was carried out in the tea garden of Guizhou Tea Research Institute (1110.83m above sea level). Spray Trichoderma asperellum SX-KXTM-T11 fermented broth of Trichoderma asperellum SX-KXTM-T11 on the surface of the tea tree by conventional spraying method, with spraying water as the control, the dosage is 8L, repeated 3 times, the area of ​​the plot is about 33m 2 , a total of about 150 tea trees were arranged in random blocks.

[0044] 1. RNA library construction and sequencing:

[0045] Using clean water as the control, tea tree leaves treated with Trichoderma asperellum SX-KXTM-T11 fermentation liquid were used as materials, and Chengdu Nomi Metabolic Biotechnology Co., Ltd. was commissioned to use the second-generation sequencing technology based on Illumina sequencing The platform completes ...

Embodiment 3

[0084] This embodiment is the influence of Trichoderma asperellum SX-KXTM-T11 fermented liquid on green tea output and quality for Trichoderma aculeatus SX-KXTM-T11:

[0085] Trichoderma asperellum SX-KXTM-T11 Trichoderma asperellum SX-KXTM-T11 fermented liquid was sprayed on the tea canopy for 30 days, and one bud and 2 leaves were collected in a 50cm×50cm sample frame. After the tea sample was brought back to the laboratory, it was first treated with a microwave oven. , medium heat, 90s; then placed in an oven at 80°C for drying treatment, and the contents of the dried samples were tested for tea quality by the High Performance Liquid Phase method by the Guizhou Tea Research Institute.

[0086] After Trichoderma asperellum SX-KXTM-T11 Trichoderma asperellum SX-KXTM-T11 fermented liquid treated the tea tree, the green tea yield showed an increasing trend compared with the clear water control (CK) (Table 7), and its increasing rate was 16.57% respectively; Compared with the wa...

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Abstract

The invention provides a trichoderma asperellum strain. The trichoderma asperellum strain is named as trichoderma asperellum SX-KXTM-T11 Trichoderma asperellum SX-KXTM-T11, the preservation number of the trichoderma asperellum strain is CCTCC NO: M2021919, the preservation unit of the trichoderma asperellum strain is China Center for Type Culture Collection, and the preservation date of the trichoderma asperellum strain is July 21, 2021. The invention further provides application for improving the disease resistance of tea trees and improving the yield of tea leaves and the quality of tea leaves. According to the invention, the disease resistance of tea trees can be improved, and the yield of tea leaves and the quality components of tea leaves are improved.

Description

technical field [0001] The invention belongs to the technical field of Trichoderma aculeatus, and in particular relates to a Trichoderma aculeatus strain and an application thereof. Background technique [0002] Tea wheel spot disease is an important leaf disease in tea cultivation and production, which has a great impact on tea tree vigor, tea quality and yield. For a long time, chemical pesticides have been an important plant protection measure in the tea planting industry. However, as the occurrence of tea garden diseases has become more and more serious, the frequency of pesticide use has also gradually increased, which has led to increasingly prominent problems such as 3R, which seriously affects the quality and safety of tea products. Tea garden ecological security. Finding safer and more effective prevention and control measures to replace the use of traditional pharmaceuticals is of great significance for promoting tea farmers' savings and efficiency, tea garden eco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/38A01P3/00A01P21/00C12R1/885
CPCA01N63/38
Inventor 赵兴丽卢声洁张莉周玉锋
Owner GUIZHOU TEA RES INST
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