Nano ofloxacin preparation and preparation method and application thereof

A technology of ofloxacin and preparations, which is applied in the field of nano-ofloxacin preparations and preparations, can solve problems such as the reduction of antibiotic efficiency, and achieve the effects of strengthening antibacterial effects, simple and mild preparation methods, and easy large-scale application

Pending Publication Date: 2021-11-09
欧青
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the overuse of antibiotics has led to the emergence of multidrug resistant (MDR) bacteria, which makes most antibiotics less effective
In addition, high-dose antibiotic therapy has a great potential to induce serious adverse reactions and systemic toxicity, such as angioedema, thrombophlebitis, anaphylactic shock, etc.

Method used

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  • Nano ofloxacin preparation and preparation method and application thereof
  • Nano ofloxacin preparation and preparation method and application thereof
  • Nano ofloxacin preparation and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] A nano-ofloxacin preparation of the present invention, the nano-ofloxacin preparation includes hollow mesoporous Prussian blue, ofloxacin and hyaluronic acid, ofloxacin loaded in the hollow mesoporous Prussian blue pores, transparent The hyaluronic acid is wrapped on the surface of the Prussian blue to seal the pores, and the nano-ofloxacin preparation is a nano-composite material of ofloxacin loaded on the Prussian blue, and the particle size of the nano-composite material is 80nm-110nm.

[0036] A preparation method of the above-mentioned nanometer ofloxacin preparation of the present embodiment, comprises the following steps:

[0037] (1) Dissolve 264mg of potassium ferricyanide and 3g of polyvinylpyrrolidone in 40mL of 0.01M HCl solution, the pH of the HCl solution is 2, then heat the resulting yellow mixture in an oil bath at 80°C for 20 hour, then the reaction gained product solution was centrifuged for 15min at a rotating speed of 12000rpm, the sediment was resus...

Embodiment 2

[0045] An application of the nano-ofloxacin preparation of the present invention in the preparation of photothermal antibacterial drugs adopts the nano-ofloxacin preparation prepared in Example 1.

[0046] like Figure 5 As shown, 100 μL of hollow mesoporous Prussian blue dispersion with a concentration of 100 μg / mL, 10 μg / mL ofloxacin and 100 μg / mL nano-ofloxacin preparation dispersion (both dispersed with deionized water) were used to treat formazan-resistant Oxicillin Staphylococcus aureus 30min, the group that near-infrared light is irradiated is irradiated with 1W / cm2, 808nm near-infrared light (NIR) for 5min, the result shows that the independent nanometer ofloxacin preparation can only kill 27.76% of bacteria, However, nano-ofloxacin preparation combined with near-infrared light treatment almost completely eradicated bacteria, and the antibacterial efficiency reached 99.99%. Image 6 The scanning electron microscope results of the bacteria showed that the morphology of...

Embodiment 3

[0048] An application of the nano-ofloxacin preparation of the present invention in inhibiting the generation of bacterial drug resistance adopts the nano-ofloxacin preparation prepared in Example 1.

[0049] Using 100 μL of PBS with a concentration of 100 μg / mL, 10 μg / mL ofloxacin and 100 μg / mL nano-ofloxacin preparation dispersion combined with laser treatment, the results are as follows Figure 7 As shown, after five cycles of culture, ofloxacin has produced drug resistance, while nano-ofloxacin preparation dispersion combined with laser treatment did not produce drug resistance.

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Abstract

The invention relates to a nano ofloxacin preparation and a preparation method and application thereof. The nano ofloxacin preparation comprises hollow mesoporous prussian blue, ofloxacin and hyaluronic acid, the ofloxacin is loaded in pores of the hollow mesoporous prussian blue, the surface of the prussian blue is wrapped with the hyaluronic acid, the pores are encapsulated, the nano ofloxacin preparation is a nano composite material of the prussian blue loaded the ofloxacin, the particle size of the nano composite material is 80-110 nm, the invention further provides the preparation method. The preparation method comprises preparation of the hollow mesoporous prussian blue, ofloxacin loading and hyaluronic acid wrapping, the nano ofloxacin preparation has a good photothermal effect, can be effective sterilization, bacteria is not easy to produce drug resistance, the effect is quick, the preparation method is simple, mild, short in time consumption, low in energy consumption and easy to realize large-scale production, and can be applied to preparation of photo-thermal treatment antibacterial drugs or traumatic infection treatment drugs.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a nano-ofloxacin preparation, a preparation method and an application. Background technique [0002] Over the past few decades, the continuous increase in bacterial infections has had serious consequences for public health worldwide. Clinically, the most widely used and most effective treatment for bacterial infections is antibiotic therapy. However, the overuse of antibiotics has led to the emergence of multidrug-resistant (MDR) bacteria, which makes most antibiotics less effective. In addition, high-dose antibiotic therapy has a great potential to induce serious adverse reactions and systemic toxicity, such as angioedema, thrombophlebitis, anaphylactic shock, etc. Therefore, there is an urgent need to develop new effective antibacterial strategies to rationally use conventional antibiotics and minimize antibiotic doses. [0003] Recently, nanoparticle (NP)-based topical ...

Claims

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Application Information

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IPC IPC(8): A61K9/52A61K47/02A61K47/36A61K9/08A61K41/00A61K31/5383A61P31/04A61P17/02A61P31/02
CPCA61K9/5115A61K9/5161A61K9/5192A61K9/0014A61K9/08A61K47/02A61K47/36A61K41/0052A61K31/5383A61P31/04A61P17/02A61P31/02A61K2300/00
Inventor 欧青
Owner 欧青
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