Quality control method of scutellaria baicalensis-perilla powder and preparation thereof
A quality control method and content technology, applied in the field of medicine, can solve problems such as complex ingredients, authenticity identification, ingredient analysis, pharmacological research and quality control difficulties, and many impurities
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[0244] (1) preparation of test sample solution: accurately weigh the effective part of the described Chinese medicine, solvent dilution and constant volume, microporous membrane filtration, get test sample solution;
[0245] (II) the reference substance solution and the test sample solution are loaded to high performance liquid chromatography and separated,
[0246] Wherein, the separation condition of described high performance liquid chromatography has the following characteristics:
[0247] (i) the chromatographic column is an octadecyl bonded silica gel column;
[0248] (ii) mobile phase is acetonitrile (A) 0.04-0.06wt% phosphoric acid aqueous solution (B);
[0249] (iii) gradient elution;
[0250] (iv) an ultraviolet detection wavelength of 250-300 nm; and
[0251] (III) Analyzing and comparing the chromatograms obtained in step (II) to obtain HPLC sample fingerprints and control fingerprints composed of their common characteristic peaks.
[0252] In the present inven...
Embodiment 1
[0310] 1.1. Prescription
[0311]
[0312] 1.2. Drug preparation and preparation methods
[0313] Take Scutellaria baicalensis medicinal material, add 8 times the amount (weight ratio) of 70% ethanol to water, heat and reflux to extract twice, each time for 2 hours, filter, and the filtrate is decompressed to recover ethanol and concentrate to a relative density of 1.05-1.20 (50-60°C) The extract was dried under reduced pressure (-0.07~-0.1MPa, ≤60°C) to obtain dry extract A.
[0314] Take flavescens medicinal material, add 10 times the amount of 50% ethanol water, heat and reflux to extract twice, each time for 2 hours, filter, and the filtrate is decompressed to recover ethanol and concentrate to a concentrated concentration with a relative density of 1.02-1.12 (50-60°C). liquid. Dilute the concentrated solution with purified water to form a dispersion (containing 0.05kg / L of crude drug), take the dispersion and pass it through a neutral alumina column, elute with 8 tim...
Embodiment 2
[0320] HPLC fingerprint and similarity analysis
[0321] 2.1 Qinsu Capsule HPLC Fingerprint Construction Method
[0322] 2.1.1 Preparation of the test solution
[0323] Take the content of Qinsu capsule, grind it finely, take about 50mg, accurately weigh it, put it in a 25ml brown measuring bottle, add 20ml of 75% methanol, ultrasonicate for 10 minutes (power 250kw, frequency 50KHz), cool to room temperature, add 75% Methanol to the mark, shake well, pass through a 0.45μm microporous membrane, and take the subsequent filtrate to obtain.
[0324] 2.1.2 Chromatographic conditions
[0325] Waters Xbridge Shield RP18 chromatographic column (4.6mm×250mm, 5μm) with octadecyl bonded silica gel as filler; mobile phase: acetonitrile (A)-0.05% phosphoric acid water (B) gradient elution, the gradient conditions are shown in Table 1 ; Detection wavelength: 270nm; Flow rate: 0.8ml / min; Column temperature: 30°C; Running time: 105 minutes (run at the initial concentration for 10 minutes t...
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