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Method for purifying aqueous solution of deacetoxycephalosporin C

An aqueous solution and purity technology, applied in the field of biochemistry, can solve problems such as increased production cost, low DAOC purity, affecting conversion yield and finished product quality, etc.

Pending Publication Date: 2021-11-12
YILI CHUANNING BIOTECH CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] DAOC (Desacetoxycephalosporin C), as an important raw material for the enzymatic preparation of 7-ADCA (7-aminodesacetoxycephalosporin C), is a metabolite of microbial fermentation. DAOC fermentation broth is filtered through multiple stages The impurity content is higher in the filtrate that produces, and DAOC purity is lower, if do not further process, reduce impurity, improve purity, will affect follow-up conversion yield and finished product quality, and can cause production cost to increase, so urgently need a kind of can to DAOC material Liquid removal and purification methods to improve subsequent production yield and product quality
[0004] And still there is no bibliography to carry out the method for purifying DAOC feed liquid at present

Method used

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  • Method for purifying aqueous solution of deacetoxycephalosporin C
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  • Method for purifying aqueous solution of deacetoxycephalosporin C

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for purifying DAOC aqueous solution with adsorption resin, specifically comprises the following steps:

[0029] (1) The DAOC aqueous solution with a potency of 8000 μg / mL was adjusted to pH 2.8 with sulfuric acid, and the temperature was controlled at 15°C;

[0030] (2) according to the adsorption capacity of 35g / L, carry out adsorption treatment through DM700 macroporous adsorption resin at the flow rate of 0.4BV / h to obtain the macroporous adsorption resin containing DAOC;

[0031] (3) washing with 4BV deionized water through the macroporous adsorption resin containing DAOC in step (1) at a flow rate of 0.5BV / h;

[0032] (4) desorb 0.5% sodium bicarbonate solution with 3.5BV concentration through step (3) macroporous adsorption resin containing DAOC at a flow rate of 0.5BV / h, and collect the desorbed liquid;

[0033] After testing, the purity of the desorbed liquid DAOC in the above embodiment was 96.97%, and the desorption rate was 92.11%.

Embodiment 2

[0035] A method for purifying DAOC aqueous solution with adsorption resin, specifically comprises the following steps:

[0036] (1) The DAOC aqueous solution with a potency of 9000 μg / mL was adjusted to pH 2.7 with sulfuric acid, and the temperature was controlled at 17°C;

[0037] (2) according to the adsorption capacity of 36g / L, carry out adsorption treatment through DM700 macroporous adsorption resin at the flow rate of 0.5BV / h to obtain the macroporous adsorption resin containing DAOC;

[0038] (3) washing with 4.5BV deionized water at a flow rate of 0.6BV / h through the macroporous adsorption resin containing DAOC in step (1);

[0039] (4) desorb the 0.4% sodium bicarbonate solution with 3.8BV concentration through step (3) the macroporous adsorption resin containing DAOC at a flow rate of 0.6BV / h, and collect the desorbed liquid;

[0040] After testing, the purity of the desorbed liquid DAOC in the above embodiment was 97.19%, and the desorption rate was 91.82%.

Embodiment 3

[0042] A method for purifying DAOC aqueous solution with adsorption resin, specifically comprises the following steps:

[0043] (1) The DAOC aqueous solution with a potency of 10,000 μg / mL was adjusted to pH 2.9 with sulfuric acid, and the temperature was controlled at 20°C;

[0044] (2) according to the adsorption capacity of 37g / L, carry out adsorption treatment through DM700 macroporous adsorption resin at the flow rate of 0.55BV / h to obtain the macroporous adsorption resin containing DAOC;

[0045] (3) washing with 4.5BV deionized water at a flow rate of 0.5BV / h through the macroporous adsorption resin containing DAOC in step (1);

[0046] (4) desorb 3.0BV concentration of 0.45% sodium bicarbonate solution through step (3) macroporous adsorption resin containing DAOC at a flow rate of 0.4BV / h, and collect the desorbed liquid;

[0047] After testing, in the above embodiment, the purity of the desorbed liquid DAOC is 97.13%, and the desorption rate is 91.87%.

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Abstract

The invention provides a method for purifying an aqueous solution of deacetoxycephalosporin C. The method comprises the following steps: adsorbing a DAOC aqueous solution through macroporous resin, washing with water, and desorbing to obtain high-purity DAOC. The method for purifying the DAOC aqueous solution is short in technological process, easy to operate, high in yield, high in purity, stable in quality and low in cost, high-purity DAOC can be obtained, and the method has very high industrial application value.

Description

technical field [0001] The invention belongs to the field of biochemistry, and in particular relates to a method for purifying an aqueous solution of deacetoxycephalosporin C. Background technique [0002] 7-ADCA (7-aminodeacetoxy cephalosporanic acid) as a new intermediate of cephalosporin antibiotics, used in the pharmaceutical industry to synthesize cefalexin, cefradine and cefadroxil and other drugs, these drugs are the market dosage larger antibiotics. [0003] DAOC (Deacetoxycephalosporin C) is an important raw material for the current enzymatic preparation of 7-ADCA (7-aminodeacetoxycephalosporanic acid), which is a metabolite of microbial fermentation. The DAOC fermentation broth is filtered through multiple stages. The impurity content in the generated filtrate is relatively high, and the purity of DAOC is relatively low. If it is not further processed, the impurities are reduced and the purity is improved, which will affect the subsequent conversion yield and the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D501/22C07D501/12
CPCC07D501/22C07D501/12
Inventor 朱洪生高旋阮卫国苏彦波杨勇王树林王瑞刘建杨伟尼米夏·巴特次仁
Owner YILI CHUANNING BIOTECH CO
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