Methylated molecular marker or combination thereof for detecting benign and malignant pulmonary nodules and application of methylated molecular marker or combination thereof

Abstract

The invention provides a DNA (deoxyribonucleic acid) methylated molecular marker for detecting benign and malignant pulmonary nodules. The DNA methylated molecular marker is any one or a combination of more than two selected from sequences as shown in SEQ ID NO. 1 to SEQ ID NO. 21 or continuous fragments of at least 55% of the total length of the sequences as shown in SEQ ID NO. 1 to SEQ ID NO. 21; or is any one or a combination of more than two selected from complete complementary sequences of the sequences shown as SEQ ID NO. 1 to SEQ ID NO. 21 or continuous fragments of at least 55% of the full length of the complete complementary sequences of the sequences shown as SEQ ID NO. 1 to SEQ ID NO. 21. The invention also provides a detection kit and detection method of the DNA methylated molecular marker. The DNA methylated molecular marker is highly related to a lung cancer, and particularly when the DNA methylated molecular marker is used in combination, the sensitivity and specificity of detecting the benign and malignant pulmonary nodules can be further improved, the detection rate of malignant pulmonary nodules is increased, and the false positive rate of detection is reduced. Primers and probes in the kit overcome the defect that a plurality of primers and probes interfere with one another when multiple PCR amplification and detection are carried out, and the quantitative performance is equal to that of single-region quantification.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a methylated molecular marker for detecting benign and malignant pulmonary nodules, its combination and application, and the application includes a detection kit and a detection method. Background technique [0002] Pulmonary nodules, that is, solitary pulmonary nodules (solitary pulmonary nodules) refer to images that are similar to circular shadows, single, well-defined, with a diameter of less than or equal to 3 cm, surrounded by high and low density of air-containing lung tissue Solid or subsolid lesions without atelectasis, hilar enlargement, or pleural effusion. [0003] Pulmonary nodules are divided into two types, benign and malignant, and often have no obvious symptoms. Benign nodules need to be treated according to the cause, while malignant nodules need early surgery. Benign etiologies are often associated with autoimmune diseases or various infections, and mal...

AI Technical Summary

Problems solved by technology

[0008] With the popularity of multi-slice spiral CT and high-resolution CT, and the improvement of people's health awareness, more and more pulmonary nodules have been detected, which is a good thing for patients, early lung cancer can be found, but it will also It causes a lot of trouble, and even many patients will feel uneasy in the bedroom after finding pulmonary nodules. Therefore, it is important to distinguish between benign and malignant pulmonary nodules in time.

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