Method for detecting quality standard of plant-derived traditional Chinese medicines in compound donkey-hide gelatin pulp

Abstract

The invention discloses a method for detecting the quality standard of plant-derived traditional Chinese medicines in compound donkey-hide gelatin pulp. The method comprises the following steps: (1) performing thin-layer chromatography identification on red ginseng and codonopsis pilosula in the compound donkey-hide gelatin pulp by adopting a one-plate multi-identification method; (2) performing thin-layer chromatography identification on prepared rehmannia root and hawthorn in the compound colla corii asini pulp by adopting a one-plate multi-identification method; (3) determining the HPLC-DAD fingerprint spectrum of the compound donkey-hide gelatin pulp; and (4) determining the content of multiple components in the compound donkey-hide gelatin pulp. According to the method, a 4-3-2-2 mode for identifying the plant-derived traditional Chinese medicines in the compound donkey-hide gelatin pulp is established for the first time, namely a double-control method of four traditional Chinese medicines, three test solution preparation methods, two developing solvents and two controls is established, and the plant-derived traditional Chinese medicines are effectively identified. An HPLC-DAD fingerprint spectrum of the compound donkey-hide gelatin pulp is established, and components of plant-derived traditional Chinese medicines in the compound donkey-hide gelatin pulp are comprehensively represented. The method for determining the content of the multiple components of the compound donkey-hide gelatin pulp is established by taking the quality markers of charred rehmannia phenylethanoid glycoside A1, isoacteoside and lobetyolin as index components, and a scientific detection method is provided for improving the quality standard of the compound donkey-hide gelatin pulp.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine, in particular to a method for detecting the quality standard of plant-derived traditional Chinese medicine in compound donkey-hide gelatin pulp. Background technique [0002] With the rapid development of traditional Chinese medicine, the popularity and application of Chinese patent medicines are becoming wider and wider. Its safety, effectiveness and quality control are the basic requirements, and the scientific and perfect quality standards are the key factors for its development. At present, the quality control mode of traditional Chinese medicine compound is mostly limited to: the identification of some medicinal flavors in the compound, the inspection of dosage forms, and the determination of the content of a few ingredients. In the traditional identification research of traditional Chinese medicine compound preparations, different medicinal flavors need to correspond to...

AI Technical Summary

Problems solved by technology

The above medicinal flavors are prepared by different methods, and the polarities of the expansion systems are greatly different and the thin-layer plates used are also different, so it is difficult to identify them at the same time under the same development conditions.

[0006] At present, there is no control of plant-derived traditional Chinese medicine in the content determination items of the current standard of compound donkey-hide gelatin pulp, which cannot fully reflect and control the overall situation of compound donkey-hide gelatin pulp, and it is difficult to control the overall quality of compound donkey-hide gelatin pulp

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