SPR sensor, SPR sensing chip comprising SPR sensor, SPR detector and application thereof

Abstract

The invention provides an SPR sensor, an SPR sensing chip comprising the SPR sensor, an SPR detector and application thereof and belongs to the technical field of micro-fluidic chips. The SPR sensor comprises an optical glass substrate and a metal film evaporated on the surface of the optical glass substrate, and the refractive index of the optical glass substrate is greater than 1.51; and the metal film incompletely covers the optical glass substrate. The metal film of the SPR sensor can be directly combined with a reaction tank of a micro-fluidic chip to form a micro-fluidic integrated chip. With the microfluidic integrated chip provided by the invention adopted, the problems of complex instrument liquid path system and tedious maintenance mode of an existing SPR detection instrument can be solved, and multiple indexes of a single sample in a complex matrix sample can be detected conveniently, simply and quickly. The invention also provides application of the product in in-vitro diagnosis and detection, and the market blank of existing disease detection products is filled.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips, in particular to an SPR sensor, an SPR sensor chip including the SPR sensor, an SPR detector and applications thereof. Background technique [0002] Surface Plasmon Resonance (SPR) is a physical optics phenomenon. Its principle means that when light is irradiated from an optically dense medium to an optically sparse medium, when the incident angle is greater than a certain angle, the interface will occur total reflection phenomenon. If there is a metal film with a certain thickness between the two medium interfaces, then the evanescent wave generated during total reflection will interact with the free electrons in the metal film to excite the surface plasmon wave (SW) that propagates along the surface of the metal film. , SPW). When the wave vector component of the incident light along the interface direction is the same as the wave vector of the SPW, the surface plasmon resonance ph...

AI Technical Summary

Problems solved by technology

This kind of operation has the following disadvantages: 1. Each time the mechanical structure is pressed together, and the way of mechanical structure pressing will cause the deformation of the reaction flow pool of the soft material; The volume of the reaction solution in the pool is inconsistent, causing the performance of the product to decline, and in severe cases, the test will fail

2. Since it is connected with the liquid circuit system, the reaction flow pool needs complex cleaning and maintenance mode after each test, which results in too long detection time

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