Somatic mutation hypersensitivity detection method based on nucleic acid mass spectrum platform

Abstract

The invention discloses a somatic mutation hypersensitivity detection method based on a nucleic acid mass spectrum platform. The method comprises the following steps: obtaining a gene to be detected; performing analysis operation on the to-be-detected gene to obtain a to-be-detected mutation site; designing a PCR amplification primer, an SAP reaction reagent, a single base extension repression primer and a wild repression probe according to a mutation site to be detected; performing amplification operation on the to-be-detected gene to obtain a PCR product; performing SAP reaction operation on the PCR product to obtain a digestion product; performing extension reaction operation on the digestion product to obtain an extension product; performing sample application operation on the extension product to obtain sample application data; analyzing the sample application data to obtain the mutation type of the to-be-detected gene; through the mode, the method is lower in cost, shorter in detection time consumption, capable of flexibly designing mutation sites, ultrahigh in sensitivity, higher in mutation detection flux, capable of simultaneously detecting nearly hundreds of mutation types, wide in application range and applicable to different sample types.

Description

Technical field [0001] The present invention relates to the field of gene detection, and more particularly to a method based on nucleic acid mass spectrometry. Background technique [0002] With the continuous in-depth and development of human genomics, drug genomics and tumor molecular biology research, the mode of tumor treatment is taken by empirical guidance to the treatment of genetic orientation. [0003] Existing genetic testing technology mainly includes: [0004] Direct sequencing method, the advantage is that the cost is low, the test results are intuitive; its disadvantage is that the process is cumbersome, the time consumption is longer, and the sensitivity is limited. [0005] Pyrophosphate sequencing method, it is advantageous that no electrophoresis does not require fluorescent labeling of DNA fragments without electrophoresis, it can quickly and accurately determine a shorter target fragment; its disadvantage is that the sequencing length is shorter, and it is eas...

AI Technical Summary

Problems solved by technology

[0012] The present invention mainly solves the problems that the existing gene detection technology has complex detection process, takes a long time, easily causes pollution, inaccurate detection results and high cost.

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