A method for the detection of neonicotinoid pesticides and their metabolites in cerebrospinal fluid
A technology of neonicotinoids and detection methods is applied in the field of pesticide detection to achieve the effects of reliable data, good repeatability and high accuracy
Active Publication Date: 2022-07-26
SOUTH CHINA AGRI UNIV
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[0004] In view of this, the present invention proposes a detection method of neonicotinoid insecticides and their metabolites in cerebrospinal fluid, to solve or partially solve the technical problems existing in the prior art
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Embodiment 1
[0063] The embodiments of the present application provide a method for detecting neonicotinoid pesticides and their metabolites in cerebrospinal fluid, comprising the following steps:
[0064] S1. Cerebrospinal fluid should be stored in -80℃ refrigerator after sampling;
[0065] S2. Add stable isotope-labeled internal standard to 250 μL of cerebrospinal fluid sample and mix with 1.5 mL of 2% formic acid aqueous solution by volume to obtain a mixed solution;
[0066] S3, mix methanol and ammonium hydroxide solution (volume concentration 30%) with a volume ratio of 19:1 to obtain an activation solution; activate the solid phase extraction cartridge with 3 ml of activation solution, and then use water to rinse the solid phase extraction cartridge, Then pass the mixed solution through the solid phase extraction cartridge; use 2 ml of a mixed solution of methanol, formic acid, and water in a volume ratio of 5:1:44 to rinse the solid phase extraction cartridge, dry under negative pr...
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Abstract
The invention provides a method for detecting neonicotinoid insecticides and their metabolites in cerebrospinal fluid, comprising the following steps: adding a stable isotope-labeled internal standard to the cerebrospinal fluid, and then mixing it with an aqueous formic acid solution to obtain a mixed solution; The solution was passed through a solid-phase extraction cartridge, then eluted with methanol, and the eluate was reconstituted with methanol aqueous solution; the reconstituted solution was detected by high performance liquid phase triple quadrupole tandem mass spectrometry. The method for detecting neonicotinoid insecticides and their metabolites in cerebrospinal fluid of the present invention adopts a solid phase extraction method with high efficiency and good repeatability combined with high performance liquid chromatography triple quadrupole tandem mass spectrometry technology to determine neonicotinoids in cerebrospinal fluid Insecticides and their metabolites, using the isotope label method, to achieve qualitative and quantitative determination of neonicotinoid pesticides and their metabolites in samples, the detection method has high sensitivity and reliable data.
Description
technical field [0001] The invention relates to the field of pesticide detection, in particular to a method for detecting neonicotinoid pesticides and their metabolites in cerebrospinal fluid. Background technique [0002] Neonicotinoids are a new type of broad-spectrum insecticides with high efficiency, safety, good selection and low toxicity to mammals. The target of these insecticides is nicotinic postsynaptic membrane acetylcholine receptors. Mainly acts on the central nervous system of insects. Since its development in the 1990s, neonicotinoid insecticides have been widely used in 140 countries around the world and currently account for more than 25% of the global pesticide market. Based on its excellent insecticidal properties and selective toxicity, this type of insecticide is not only widely used in agricultural production, but also has a large number of applications in household life, such as lawn, home garden maintenance and management, livestock and pet pest cont...
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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 李晶魏鑫潘亚男司梦雅涂海欣张珍岩银仁莉王裕文李沛然林泽王振焦晓阳
Owner SOUTH CHINA AGRI UNIV