Recombinant I-type humanized collagen C1L1T and preparation method and application thereof

A technology of collagen and humanization, applied in the field of genetic engineering, can solve the problems of heterologous collagen immune response, such as difficult absorption and utilization, long cycle, low utilization rate of collagen, etc., achieve good cell adhesion effect, and simple preparation method Effect

Pending Publication Date: 2021-11-23
SHANXI JINBO BIO PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the fact that heterologous collagen in this field is easy to induce immune response and is not easy to be absorbed and utilized by the human body, and the low utilization rate of collagen caused by the high production cost, long cycle and inability to produce large-scale production of human collagen, this paper The invention provides a recombinant type I humanized collagen C1L1T, as well as its preparation method and use

Method used

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  • Recombinant I-type humanized collagen C1L1T and preparation method and application thereof
  • Recombinant I-type humanized collagen C1L1T and preparation method and application thereof
  • Recombinant I-type humanized collagen C1L1T and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0054] Example 1: Preparation of recombinant type I humanized collagen C1L1T

[0055] 1. Construction of C1L1T gene expression vector

[0056] The full-length protein sequence of the recombinant type I humanized collagen C1L1T used in this example is the sequence shown in SEQ ID No.4, with a full-length of 222aa, and the corresponding full-length gene is 666bp. 针对大肠杆菌的密码子进行了密码子优化,优化后的序列为:GGGCCCATGGGACCTTCAGGTCCAAGGGGATTGCCGGGTCCTCCGGGTGCTCCGGGTCCGCAGGGTTTTCAGGGTCCGCCAGGCGAGCCGGGCGAGCCGGGCGCTAGCGGTCCGATGGGTCCGCGTGGTCCGCCGGGCCCGCCGGGCAAGAACGGCGACGACGGTGAGGCAGGCAAACCGGGGCGCCCTGGTGAACGTGGTCCACCGGGTCCGCAAGGTGCGAGAGGCCTGCCCGGTACCGCAGGCCTGCCGGGCATGAAAGGTCACCGTGGCTTCAGCGGTCTGGATGGTGCGAAGGGCGACGCAGGTCCGGCGGGTCCAAAAGGCGAGCCGGGTTCCCCGGGCGAAAATGGCGCGCCTGGCCAGATGGGTCCGCGTGGTTTACCGGGCGAGCGCGGCCGTCCGGGCGCGCCGGGCCCAGCGGGTGCCCGTGGAAACGATGGCGCGACGGGTGCGGCTGGCCCACCGGGCCCGACCGGTCCGGCGGGTCCGCCGGGTTTCCCGGGTGCCGTTGGTGCGAAGGGTGAAGCAGGCCCCCAAGGTCCGCGCGGTTCTGAAGGTCCGCAAGGGGTGCGTGGTGAACCGGGCCCGCCGGGCCCGGC...

Embodiment 2

[0068] Example 2: Bioactivity Detection of Recombinant Type I Humanized Collagen C1L1T

[0069] Collagen activity detection method can refer to the literature Juming Yao, Satoshi Yanagisawa, Tetsuo Asakura, Design, Expression and Characterization of Collagen-Like Proteins Based on the Cell Adhesive and Crosslinking Sequences Derived from Native Collagens, J Biochem.136, 643-649 (2004). The specific implementation method is as follows:

[0070] (1) Use the ultraviolet absorption method to detect the concentration of the protein samples to be tested, including commercially available human collagen (Sigma, C7774), and the recombinant type I humanized collagen C1L1T provided by the present invention.

[0071] Specifically, the UV absorption of samples at 215nm and 225nm was measured respectively, and the protein concentration was calculated using the empirical formula C(μg / mL)=144×(A215-A225). Note that the detection should be performed when A215<1.5. The principle of this method...

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Abstract

The invention discloses recombinant I-type humanized collagen C1L1T and a preparation method and application thereof. The recombinant I-type humanized collagen C1L1T comprises a sequence shown as SEQ ID No. 3; optionally, the recombinant I-type humanized collagen C1L1T comprises a sequence shown as SEQ ID No. 2; and preferably, the sequence shown as SEQ ID No. 3 is directly connected with the sequence shown as SEQ ID No. 2. The recombinant I-type humanized collagen C1L1T has the advantages that the activity of promoting cell adhesion is realized, and the amino acid sequence of the recombinant I-type humanized collagen C1L1T is selected from the amino acid sequence of natural I-type human collagen; and when the recombinant I-type humanized collagen C1L1T is applied to a human body, the immune reaction is avoided; the preparation method is simple, and the collagen with higher yield can be obtained at low cost.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering and relates to a recombinant type I humanized collagen C1L1T and its preparation method and application. Background technique [0002] Collagen is a protein family that is abundant in animals and widely distributed in animal skin, bones, tendons, ligaments and blood vessels. It is the most abundant protein in mammals and is also a component of the extracellular matrix. important constituents. Collagen accounts for 25% to 30% of the total protein in the human body, plays an important role in protecting and connecting various tissues, and plays an important physiological function in the body. Type I collagen is the most abundant in the human body, accounting for more than 85%. Type I collagen is high in bone, skin, tendon and cornea; Type II exists in cartilage, intervertebral disc and vitreous body; Type III exists in blood vessels, In new skin and scar tissue. [0003] Collagen has ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C07K1/22C07K1/18C12N15/12C12N15/62C12N15/70C12N1/21A23L33/18A61K38/39A61P43/00A61K8/65A61Q19/00C12R1/19
CPCC07K14/78C12N15/70A23L33/18A61P43/00A61K8/65A61Q19/00C12N2800/22C07K2319/50A23V2002/00A61K38/00A61K2800/10A23V2200/30A23V2250/55
Inventor 杨霞武庚风张永健
Owner SHANXI JINBO BIO PHARMA CO LTD
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