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Polypeptide for promoting skin repair and application thereof

A nucleic acid and selected technology, applied in skin repairing peptides and its application fields, can solve the problems of insufficient stability and dispersibility of cosmetics, achieve the effects of promoting collagen expression, anti-oxidation, good stability, and promoting skin repair Effect

Inactive Publication Date: 2021-11-26
四川丽妍工坊生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] These polypeptides all have the function of promoting skin repair, but they have many shortcomings in the application of pharmaceuticals and cosmetic products, such as the inability to apply to the preparation of cosmetics or the defects of insufficient stability and dispersibility when preparing cosmetics, and cannot effectively play their roles in promoting skin repair. The effect of skin repair

Method used

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  • Polypeptide for promoting skin repair and application thereof
  • Polypeptide for promoting skin repair and application thereof
  • Polypeptide for promoting skin repair and application thereof

Examples

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preparation example Construction

[0048] The embodiment of the present invention also provides a preparation method of a polypeptide, which comprises: cultivating the host cell as described in the foregoing embodiments, or artificially synthesizing the polypeptide as described in any of the foregoing embodiments.

[0049] Conditions for culturing host cells can be obtained based on conventional techniques, as long as the host cells can express polypeptides, and will not be repeated here.

[0050] The embodiments of the present invention also provide the use of the polypeptide as described in any of the foregoing embodiments or the isolated nucleic acid as described in the foregoing embodiments in the preparation of a drug or cosmetic that promotes repair of damaged skin.

[0051] The embodiments of the present invention also provide the use of the polypeptide as described in any of the foregoing embodiments or the isolated nucleic acid as described in the foregoing embodiments in the preparation of drugs or cos...

Embodiment 1

[0060] The present embodiment provides skin repair promoting polypeptide RW3, which is a linear polypeptide, and the primary structure of the complete amino acid sequence is: Met-Pro-Phe-Arg-Met-Gly-Ile-Cys-Thr-Met-Asn (MPFRMGICTMN ).

[0061] The preparation method of the skin-promoting peptide RW3, the specific steps are as follows:

[0062] 1. According to the designed amino acid sequence: Met-Pro-Phe-Arg-Met-Gly-Ile-Cys-Thr-Met-Asn, the crude polypeptide was synthesized by solid phase synthesis;

[0063] 2. Desalting and purifying the crude polypeptide by HPLC reverse-phase column chromatography, and identifying its purity until the purity of the polypeptide is not less than 95%;

[0064] HPLC purification and identification method: Dissolve 0.1mg of the sample to be tested in 1mL ultrapure water containing 0.1% trifluoroacetic acid. If there are undissolved impurities, filter them with a 0.45μm filter membrane. Mobile phase A is 0.1% trifluoroacetic acid - water, the mo...

Embodiment 2-8

[0076] Examples 2-8 respectively provide a polypeptide and its preparation method. The preparation method is roughly the same as that of Example 1, the difference is that the sequence is different. The amino acid sequence of the polypeptide provided by Examples 2-8 is as follows:

[0077] RW0: AMFRMGICTMN (SEQ ID No. 1);

[0078] RW1: AMFRMGMCTMN (SEQ ID No. 2);

[0079] RW2: APFRMGICTMN (SEQ ID No. 3);

[0080] RW4: MPFRMGMCTMN (SEQ ID No. 5);

[0081] RW5: MPFRMGMCTMM (SEQ ID No. 6);

[0082] RW6: PFRMGMC™ (SEQ ID No. 7);

[0083] RW7: PFRMGMCTMN (SEQ ID No. 8).

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Abstract

The invention discloses a polypeptide capable of promoting skin repair and application thereof, and relates to the technical field of biological medicine, in particular to a polypeptide capable of promoting skin repair, the proportion of hydrophobic amino acid in the polypeptide capable of promoting skin repair is 16-30%, the isoelectric point of the polypeptide is 0.55-1.00, the sequence length is 7-14aa, and the polypeptide capable of promoting skin repair has a skin repair function. By reasonably designing the proportion of hydrophobic amino acids, isoelectric points and appropriate peptide chain length, the polypeptide can maintain good stability in both acidic and alkaline solvents, has strong functions of promoting skin repair, promoting collagen expression, resisting oxidation and the like, and the polypeptide can be applied to preparation of cosmetics and medicines with the function of promoting skin repair.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a polypeptide for promoting skin repair and its application. Background technique [0002] The skin is the largest organ covering the surface of the human body, and is easily affected by many factors, such as external physical and chemical factors, changes in its own hormone levels, and mechanical pressure. At the same time, the skin is the first natural barrier between the body and the outside world. Once damaged, the body will immediately start physiological regulation to repair the skin damage. Skin restoration has long been a common problem in clinical surgery. [0003] There are two types of cosmetics currently on the market: one is cosmetics with chemical raw materials as the main component, and the other is cosmetics with plant raw materials as the main component. Chemical cosmetics are effective quickly, but they contain toxic and harmful substances such as hormones...

Claims

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Application Information

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IPC IPC(8): C07K7/06A61K38/08A61P17/02A61K8/64A61Q19/08A61Q19/00
Inventor 何阳
Owner 四川丽妍工坊生物科技有限公司
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