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Method for determining very low density lipoprotein (VLDL) subcomponents and component distribution thereof

A very low density and component distribution technology, applied in the biological field, it can solve the problems of non-recognized normal range of measurement results, large individual fluctuations, and inability to be effectively applied.

Pending Publication Date: 2021-12-03
PROTEINT (TIANJIN) BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Using methods such as ultracentrifugation or nuclear magnetic resonance, the total VLDL can be divided into subcomponents according to the density, and VLDL can be divided into 2-5 components, and the component content (triglyceride, triglyceride, Cholesterol, free cholesterol, phospholipids), but there is no recognized normal range for the measurement results, and individual fluctuations are large, so it cannot be effectively used

Method used

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  • Method for determining very low density lipoprotein (VLDL) subcomponents and component distribution thereof
  • Method for determining very low density lipoprotein (VLDL) subcomponents and component distribution thereof
  • Method for determining very low density lipoprotein (VLDL) subcomponents and component distribution thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 The NMR method was used to compare the distribution characteristics of VLDL esters in healthy people, before and after liver cancer surgery.

[0025] 1) Sample: This method detects the blood collected on an empty stomach. After the collected blood is centrifuged, the precipitated blood cells are discarded, and the upper plasma / serum sample is taken. The test can accept fresh blood or separated or frozen serum / plasma, blood sample Hemolysis should not occur. In order to maintain the consistency of test results, the same blood collection tube should be used for multiple test results of the same individual.

[0026] 2) Sample buffer preparation: Sodium dihydrogen phosphate buffer 0.075mol / L, deuterated water 20%, 0.03% TSP, prepared with pure water, fully dissolved and mixed, subpackaged and refrigerated. Preheat to room temperature.

[0027] 3) Sample pretreatment: put fresh or completely melted serum / plasma at room temperature to return to temperature and sha...

Embodiment 2

[0038] Example 2 Ultracentrifugation was used to compare the distribution characteristics of VLDL esters in healthy people, before and after liver cancer surgery.

[0039] 1) Sample: This method detects the blood collected on an empty stomach. After the collected blood is centrifuged, the precipitated blood cells are discarded, and the upper plasma / serum sample is taken. The test can accept fresh blood or separated or frozen serum / plasma, and blood samples Hemolysis should not occur. In order to maintain the consistency of test results, the same blood collection tube should be used for multiple test results of the same individual.

[0040] 2) Sample buffer: EDTA solution (0.5mol / L): Add 950ml of deionized water to 186g EDTA-Na2, adjust the pH value with NaOH, and add water to 1L. Density solution: NaCl 11.40g, 0.5mL 0.5mol / L EDTA solution, add deionized water to 1L.

[0041] 3) Sample pretreatment: Take 3 mL of plasma, add 1.5 mL of density solution, and use ultracentrifugat...

Embodiment 3

[0045] Example 3 The NMR method was used to compare the distribution characteristics of VLDL esters in healthy people and liver cancer before and after radiotherapy.

[0046] 1) Sample: This method detects the blood of healthy people collected on an empty stomach, patients before and after radiotherapy for liver cancer, the collected blood is centrifuged, the precipitated blood cells are discarded, and the upper plasma / serum sample is taken for testing. Fresh blood or Separated or frozen serum / plasma.

[0047] 2) Sample pretreatment and detection method: the same as in Example 1.

[0048] 3) Index extraction and calculation: Same as Example 1.

[0049] The statistical results of the measured data in different populations are as follows:

[0050] .

[0051] The statistical results of calculating the proportion of ingredients in different groups of people are as follows:

[0052] .

[0053] Take the first and fifth components of VLDL as an example (VLDL-1, VLDL-5), such...

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Abstract

The invention discloses a method for determining very low density lipoprotein (VLDL) subcomponents and component distribution thereof. The method comprises the following steps of (1) according to density of VLDL, dividing the total VLDL into 2-5 subcomponents, and determining contents of triglyceride, cholesterol, free cholesterol and phospholipid in each subcomponent; (2) calculating the ratio of triglyceride in each sub-component to total triglyceride in each sub-component, and calculating the ratio of cholesterol, free cholesterol and phospholipid according to the same method to obtain the distribution condition of the VLDL component in each sub-component; and (3) according to the individual time / intervention process, carrying out multiple times of measurement, and comparing the change condition of the distribution of each component. When individual diseases or abnormal lipid metabolism occur and the detection result of the total very low density lipoprotein is stable, the method can reflect the change state of the individual lipid metabolism according to the distribution change rule of the triglyceride, the cholesterol, the free cholesterol and the phospholipid in the subfractions.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for measuring very low-density lipoprotein subcomponents and component distribution thereof. Background technique [0002] The liver is an important organ for fat metabolism, and very low-density lipoprotein (VLDL) is the most important carrier of lipid metabolism in the liver: very low-density lipoprotein is an intermediate between chylogranules, remnants, bile acids, fatty acids, sugars and proteins used by the liver A lipoprotein composed of metabolites and apolipoproteins synthesized in the liver. The main function of VLDL is to transport endogenous triglycerides synthesized in the liver. Whether it is the fatty acid transported by the blood to the liver cells or the fatty acids formed by the transformation of sugar metabolism, triglycerides can be synthesized in the liver cells, and finally transported by VLDL to the extrahepatic organs to perform the lipid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/25G01N21/59G01N24/08
CPCG01N21/25G01N21/59G01N24/082
Inventor 李捷任丽秦迪
Owner PROTEINT (TIANJIN) BIOTECHNOLOGY CO LTD
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