Method for simulating dynamic digestion of gel food based on artificial gastrointestinal system

Abstract

The invention relates to a method for simulating dynamic digestion of gel food based on an artificial gastrointestinal system. The method comprises the following steps: preparing digestive juice, treating a protein gel sample, simulating dynamic gastrointestinal digestion and collecting digestion products. Compared with static digestion, dynamic digestion can better simulate the change of protein gel food in the digestive tract of the human body, and especially in the gastric digestion stage, the dynamic digestion can digest protein more thoroughly. By utilizing the bionic gastrointestinal device, dynamic secretion of digestive juice in the digestion process, real-time adjustment of the pH value of the digestion environment, increase of dynamic simulation of peristalsis extrusion and continuous gastric emptying of a digestion sample and the like are realized, so that an experimental result is more real and reliable. Aiming at a unique structure of protein gel food, dynamic digestion can simulate a human digestion environment more truly, and compared with static digestion, protein in protein gel can be digested better and more thoroughly in a dynamic digestion model, especially in a gastric digestion stage.

Description

technical field [0001] The invention relates to the technical field of Chinese food measurement, and relates to a method for simulating dynamic digestion of gel food based on an artificial gastrointestinal system. Background technique [0002] Protein gel food is very common in daily life and has a wide variety of products. It is loved by consumers because of its unique taste and high nutritional value, such as surimi gel, cheese, and tofu. With the transformation of people's consumption concept and the continuous improvement of the pursuit of healthy food, it is necessary to evaluate the digestion and absorption characteristics of food. Common digestion methods are divided into in vitro simulated digestion and animal in vivo digestion. Although in vivo digestion can more truly reflect the changes of food in the gastrointestinal tract, it is limited by the ethical constraints of experimental animals, high cost, and cumbersome experimental procedures. Therefore, in vitro sim...

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Problems solved by technology

Although in vivo digestion can more truly reflect the changes of food in the gastrointestinal tract, it is limited by the ethical constraints of experimental animals, high cost, and cumbersome experimental procedures. Therefore, in vitro simulated digestion methods are often used

In vitro simulated digestion is divided into static digestion and dynamic digestion. Although static digestion has the advantages of simplicity and low cost, it can simulate the chemical environment and enzymatic environment in the digestion process to a certain extent. Equipment such as magnetic stirrers or water bath oscillators used in digestion can only simply mix and stir food and digestive juices, and cannot simulate the damage caused by gastrointestinal peristalsis and contraction to food. Therefore, it is necessary to propose an artificial gastrointestinal system based on The method of simulating the dynamic digestion of protein gel foods provides a reference for exploring the digestive characteristics of protein gel foods

Although the Chinese invention patent CN112837590A utilizes an artificial stomach model, it is only suitable for liquid food and infants, and is limited to gastric digestion

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